外周血中BAFF、BAFF-R及Bcl-xL的表达与B细胞非霍奇金淋巴瘤疾病进展的相关性研究

Correlation between expressions of BAFF,BAFF-R and Bcl-xL in peripheral blood and progression in B cell non-Hodgkin′s lymphoma patients

目的研究B细胞性非霍奇金淋巴瘤(B-NHL)患者外周血B cell-activating factor(BAFF)、其受体BAFF-R及Bcl-xL基因的表达,探讨其与B-NHL疾病进展的相关性。方法应用实时荧光定量PCR法检测36例B-NHL患者外周血单个核细胞(PBMC)BAFF、BAFF-R、Bcl-xL mRNAs的相对表达水平,以7例健康献血员作为对照,并据性别、淋巴瘤国际预后指数(IPI)、年龄、临床分期、是否耐药进行分组比较。结果 B-NHL患者PBMC BAFF、BAFF-R及Bcl-xL的mRNAs表达较正常对照组显著升高(P<0.05),Ⅲ/Ⅳ期高于Ⅰ/Ⅱ期,耐药B-NHL组高于非耐药组(P<0.05)。结论外周血单个核细胞BAFF、BAFF-R、Bcl-xL mRNAs的表达水平与B-NHL临床分期有关,在耐药B-NHL外周血中明显升高,提示BAFF/BAFF-R介导的信号通路可能参与B-NHL的临床进展及耐药机制。

Objective To detect the expressions of BAFF,BAFF-R and Bcl-xL in peripheral blood from patients with B cell non-Hodgkin′s lymphoma（B-NHL）,and to investigate the correlation between the level of these genes and the progression of B-NHL.Methods The expression of BAFF,BAFF-R and Bcl-xL in peripheral blood mononuclear cells（PBMC） from patients（n=36） and healthy volunteers（n=7） was examined via real-time fluorescence quantitative polymerase chain reaction（real-time q-PCR） at the mRNAs level.These data were further analyzed according to gender,lymphoma international prognostic index（IPI）,age,clinical staging and drug resistance.Results The expressions of BAFF,BAFF-R and Bcl-xL were significantly increased in PBMC from patients with B-NHL compared with control group（P0.05）.The expression of these genes in groups of stage Ⅲ/Ⅳ and the resistant B-NHL was respectively higher than that of stageⅠ/Ⅱ and non resistant B-NHL groups（P0.05）.Conclusion The expression of BAFF,BAFF-R and Bcl-xL in PBMC from patients with resistant B-NHL is significantly increased,correlated with the B-NHL clinical staging.These results suggest that BAFF/BAFF-R mediated signal pathway is involved in the progression of B-NHL and drug resistance.