白纹伊蚊及致倦库蚊对蝙蝠乙型脑炎病毒分离株的易感性分析

Susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to infection with bat Japanese encephalitis virus isolates

目的了解蝙蝠乙脑病毒分离株GD1和HN2株对白纹伊蚊和致倦库蚊的易感性。方法通过病毒液饲喂法使白纹伊蚊和致倦库蚊经口感染蝙蝠乙脑病毒GD1株和HN2株,采用TaqMan real-time RT-PCR方法,检测GD1株和HN2株感染白纹伊蚊及致倦库蚊后第4、6、8、10、12、14、16、18、20天病毒存在情况与病毒载量的动态变化。结果两株蝙蝠乙脑病毒在感染白纹伊蚊、致倦库蚊后4~20 d内均能被检测到。Ct值的动态变化规律显示白纹伊蚊和致倦库蚊均能感染乙脑病毒GD1株和HN2株,但致倦库蚊的易感性高于白纹伊蚊;两株毒株的毒力可能不同,HN2株毒力大于GD1株。结论白纹伊蚊和致倦库蚊均能感染蝙蝠乙脑病毒分离株。

Objective To evaluate the susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to oral infection with bat Japanese encephalitis virus isolates（GD1 and HN2 strains）.Methods Aedes albopictus and Culex pipiens quinquefasciatus were infected orally by GD1 and HN2 strains of bat Japanese encephalitis virus.TaqMan real-time PCR was used to detect the virus and monitor the changes in the viral loads in Aedes albopictus and Culex pipiens quinquefasciatus at a 2-day interval,starting from 4 days till 20 days after the infection.Results The infected Aedes albopictus and Culex pipiens quinquefasciatus were found positive for the Japanese encephalitis virus from day 4 to day 20.Both Aedes albopictus and Culex pipiens quinquefasciatus were susceptible to infection by GD1 and HN2 strains,but the latter showed a greater susceptibility.The HN2 strain virus appeared to have a greater virulence than the GD1 strain.Conclusion Aedes albopictus and Culex pipiens quinquefasciatus can carry GD1 and HN2 strains of bat Japanese encephalitis virus isolates.