铜绿假单胞菌制剂抑制鼻咽癌细胞体外增殖

Pseudomonas aeruginosa vaccine inhibits the proliferation of human nasopharyngeal cancer cells in vitro

目的观察铜绿假单胞菌(PA-MSHA)注射液对人鼻咽癌细胞株5-8F及6-10B的生长抑制作用,初步探讨其作用机制。方法 MTT法检测鼻咽癌细胞体外增殖情况,流式细胞术检测细胞周期,Westem blotting检测细胞周期及凋亡相关蛋白。结果MTT结果表明,与未加药组相比,加药组5-8F及6-10B细胞的增殖能力明显降低(P<0.05),呈剂量和时间依赖性。细胞周期检测发现药物处理后的肿瘤细胞发生G1/S期阻滞(P<0.05)。Westerm blotting检测发现药物处理后的两组细胞周期相关蛋白cyclinD1、CDK4、CDK6及凋亡促进蛋白Bax表达上调,凋亡抑制蛋白Bcl-2表达下调。结论 PA-MSHA能够抑制鼻咽癌5-8F及6-10B细胞株增殖,其机制可能是通过影响细胞周期调节并促进细胞凋亡。

Objective To investigate the inhibitory effects of Pseudomonas eruginosa vaccine（PA-MSHA） on the proliferation of human nasopharyngeal cancer cells and explore the possible mechanism.Methods MTT assay was used to determine the cell growth of human nasopharyngeal cancer cell line 5-8F and 6-10B in vitro treated with the vaccine.The cell cycle distribution of the cells was detected by flow cytometry,and the expression levels of apoptosis and cycle-related proteins were evaluated by Western blotting.Results PA-MSHA treatment significantly suppressed the proliferation of 5-8F and 6-10B cells in a time-and concentration-dependent manner compared with the control group（P0.05）.The cells with PA-MSHA treatment exhibited a decreased percentage of cells entering S phase and a corresponding increase in G1 phase cells in FACS analysis.The expression of cyclin D1,CDK4,and CDK6 was significantly up-regulated,Bax protein up-regulated,and the anti-apoptosis protein Bcl-2 down-regulated in PA-MSHA-treated cells.Conclusion PA-MSHA can suppress the proliferation of nasopharyngeal carcinoma cell in vitro by affecting the cell cycle and promoting cell apoptosis.