摘要
目的探讨IBP对SACC细胞抗紫杉醇凋亡能力的影响及其机制。方法将ACC2转染IBP组和空白对照组各自根据不同紫杉醇浓度分成5组,紫杉醇作用72 h后,MTT法检测在紫杉醇作用下IBP对SACC细胞增殖的影响;通过微管蛋白Tubulin免疫荧光染色,观察紫杉醇作用前后ACC2细胞微管的变化及IBP与微管的关系。结果 IBP使ACC2细胞对紫杉醇产生一定程度的耐药,在5μg/ml的紫杉醇浓度下最为明显;紫杉醇开始作用后,ACC2-C1细胞的微管点状聚集成团,而ACC2-C1/IBP细胞的微管则出现明显的紊乱、断裂,IBP能促进微管的解聚;IBP所发的绿色荧光与微管的红色荧光糅合在一起呈黄色,IBP与微管存在一定程度的共定位。结论 IBP促进SACC细胞对紫杉醇耐药。
Objective To study the effect of IRF-4 binding protein (IBP) on resistance of salivary adenoid cystic carcinoma (SACC) cells to taxol and its mechanism. Methods ACC2 cells transfected with IBP and blank control cells (ACC2-C1 cells) were divided into 5 groups according to their taxol concentration. Effect of IBP on proliferation of SACC cells was detected by MTT assay 72 h after taxol was used. Occurrence of changes in microtubules of SACC cells and its relation with IBP were observed with tubulin immunofluorescence staining before and after taxol was used. Results IBP increased the resistance of ACC2 cells to taxol, especially at the concentration of 5 p,g/ml. The point-like microtubules of ACC2-C1 cells aggregated into a clump while the microtubules of ACC2-C1/IBP cells were arranged in disorder and fractured, indicating that IBP can promote depolymerization of microtubules. Green fluorescence of IBP and red fluorescence of microtubules were merged as a yellow color, showing that IBP and microtubules are located at the same site. Conclusion IBP promotes resistance of SACC cells to taxol.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第8期723-725,共3页
Journal of Third Military Medical University
基金
国家自然科学基金(30901458)
重庆市自然科学基金(CSTC2009BB5027)~~
关键词
IBP
紫杉醇
免疫荧光染色
TUBULIN
IRF4 binding protein
taxol
immune immunofluorescence staining
tubulin