外源性硫化氢对大鼠肝纤维化的影响

Effect of exogenous hydrogen sulfide on liver fibrosis in rats

目的探讨外源性硫化氢对四氯化碳(CCl4)诱导大鼠肝纤维化的预防作用。方法将24只Wistar雄性大鼠随机分为3组,每组8只,即正常对照组、模型组、硫化氢(H2S)预防组。H2S预防组除腹腔注射40%CCl4外,还给予NaHS腹腔注射,1次/d,至第8周,所有大鼠均被处死,收集大鼠血液,检测肝纤维化指标;取肝组织检测脂质过氧化指标及观察肝脏病理变化;应用RT-PCR和Western blot方法检测肝组织NF-κB的表达。结果外源性H2S能降低血清HA、LN、PCⅢ及肝组织MDA含量(P<0.01或P<0.05),增加肝组织SOD、GSH-Px活性(P<0.01),减轻肝纤维化程度(P<0.05),而且能使NF-κB表达明显减少。结论外源性硫化氢对CCl4诱导的大鼠肝纤维化具有保护作用,其机制可能与硫化氢抑制NF-κB表达有关。

Objective To study the preventive effect of exogenous hydrogen sulfide （H2S） on carbon tetrachloride（ CCI4）-induced hepatic fibrosis in rats. Methods Twenty-four male Wistar rats were randomly divided into normal control group （ n = 8 ）, model group （ n = 8 ）, and H2 S prevention group （ n = 8 ）. NariS was injected into the abdominal cavity of rats in HzS prevention group in addition to 40% CCI4, once a day, for 8 weeks. Then, all rats were sacrificed and blood samples were collected to test the indications of hepatic fibro- sis. Lipid peroxide indications and liver pathological changes were observed in liver tissue samples. Expression of NF-KB in liver tissue was detected by RT-PCR and Western blotting, respectively. Results Exogenous H2S significantly decreased the serum HA,LN,PC m i and MDA levels, hepatic fibrosis, expression of NF-kB and increased the SOD and GSH-Px activities in liver tissues （ P 〈 0.01 or P 〈 0.05 ）. Conclusion Exogenous H2S protects against CC14-induced hepatic fibrosis in rats by inhibiting the expression of NF-KB.