快速检测TOX、CMVIgG抗体的适配子型SPR传感器微阵列的初步构建

Construction of aptamer-based surface plasma resonance biosensor microarray for the rapid detection of toxoplasma godii and cytomegalovirus IgG antibodies

目的初步构建弓形虫(toxoplasma gondii,TOX)、巨细胞病毒(cytomegalovirus,CMV)IgG抗体的适配子型SPR传感器微阵列的快速检测方法。方法采用SELEX技术筛选TOX、CMV IgG的适配子,并将其整合于SPR生物传感器实时在线分析系统,通过在传感器表面固定探针分子,对溶液中的TOX、CMV IgG进行杂交检测,并进一步研究该检测方法的稳定性与线性检范围。结果该新型快速检测方法能够实现对TOX、CMV IgG的实时检测,检测系统稳定性良好,八通道间检测时互不影响,线性检测范围为20～300μmol/L。结论该实验建立的快速检测方法,具有稳定性好等优点,SPR传感器技术结合适配子技术在临床诊断工作中有着广阔的应用前景。

Objective To construct the aptamer-based surface plasma resonance （SPR） microarray for rapid assay of toxoplasma gondii （TOX） and cytomegalovirus （CMV） IgG antibodies. Methods Aptamers of the TOX gondii and CMV IgG antibodies were screened with the SELEX technique and integrated into the re- al-time online analyzing system on the SPR biosensor. Hybridization of TOX and CMV IgG antibodies in solution was detected according to the probe molecules fixed on the surface of biosensor. The stability and linear detec- tion range of this assay were further investigated. Results The novel rapid method could assay the TOX and CMV IgG antibodies with a good stability and a linear assay range of 20 to 300 i.tmol/L. Conclusion The sta- bility of the rapid assay we established is good. Combined SPR biosensor and aptamer techniques have a broad prospect in clinical assay of TOX and CMV IgG antibody level.