神经生长颗粒对大鼠坐骨神经挤压伤修复的影响

Effects of Nerve Growth Granules on Recovery of Scatic Nerve Crush Injury

目的:研究神经生长颗粒对大鼠坐骨神经挤压伤修复的影响。方法:雌性SD大鼠60只,麻醉后行坐骨神经夹伤术。术后第2天开始灌药,并随机分为5组:阳性对照弥可保组;实验组神经生长颗粒高、中、低剂量组;生理盐水阴性对照组。灌药后3周,通过对术后动物的整体观察,压伤段神经电生理学检测和腓肠肌组织学形态测量,评价修复效果。结果:灌药后3周,大鼠坐骨神经干复合肌动作电位(CMAP)和神经传导速度(MCV),NGG高剂量组与弥可保组比较差异无统计学意义,明显优于生理盐水组;腓肠肌肌湿重比,NGG高、中剂量组与弥可保组比较差异无统计学意义,均显著优于生理盐水组;胶原纤维面积NGG高剂量组与弥可保组比较差异无统计学意义,显著低于生理盐水组。结论:神经生长颗粒有明显的促神经再生和促神经功能恢复的作用。

Objective： To evaluate the effects of nerve growth granules on rat sciatic nerve crush injury recovery.Methods： Sixty female SD rats were randomly divided into five groups： The methycobal group as the positive control,three NGG groups（high,moderate and low dose）,and saline as the negative control.Electrophysiology and histochemistry were performed after 3 weeks of sciatic nerve crush operation.Results： The CMAP amplitude ratio and the MCV value showed no significant difference between the NGG high dose group and Methycobal group;in addition,CAMP and the MCV value amplitude ratio were significantly larger in either the NGG high dose group or the Methycobal group than in the saline group.Gastrocnemius muscle wet weight ratio and Masson trichrome indicated that the astrocnemius muscle atrophy was less serious in the NGG high dose group and the Methycobal group than in the saline group.Conclusions： NGG could promote peripheral nerve regeneration and recovery of its function.