脑星形细胞瘤中PTEN和VEGF蛋白的表达及其相关性

Expressions of PTEN and VEGF in human astrocytoma and correlation between them

目的探讨PTEN基因及血管内皮生长因子(VEGF)在脑星形细胞瘤中表达及其相关性。方法采用SP免疫组化法检测PTEN和VEGF在65例脑星形细胞瘤组织(A组)和10例正常脑组织(B组)中的表达。结果 A组PTEN表达阳性率为49.2%,明显低于B组的100%(P<0.01);A组VEGF表达阳性率为64.6%,B组无阳性表达。与低恶性度组比较,高恶性度组的PTEN低,而VEGF表达明显增高(P<0.05)。在脑星形细胞瘤组织中,PTEN与VEGF蛋白的表达呈负相关(r=-0.980,P<0.05)。结论 PTEN及VEGF均与脑星形细胞瘤发生及恶性程度密切相关;检测两者表达情况可为脑星形细胞瘤的临床诊治及预后判断提供依据。

Objective To investigate the expressions of phosphatase and tensin homology deleted on chromosome 10（PTEN） and vascular endothdial growth factor（VEGF） in human astrocytoma and the correlation between them.Menthods The expressions of PTEN and VEGF were detected by SP immunohistochemistry in 65 cases of human astrocytomas（group A） and 10 cases of normal brain tissues（group B）.Results The positive expression rate of PTEN was lower in group A than that in group B（49.23% vs.100%）（P〈0.01）.The positive expression rate of VEGF was 64.6%,which was zero in group B.The positive expression rate of VEGF in group A was higher in the cases with high-grade astrocytoma than that in those with low-grade（P〈0.05）.The positive expression of VEGF was negatively correlated to that of PTEN（r=-0.980,P〈0.05）.ConclusionBoth PTEN and VEGF proteins are closely correlated with the occurrence and differentiation of human astrocytoma.Detection of PTEN and VEGF may be heipful in the diagnosis,treatment and prognosis prediction.