圆臀大黾蝽(昆虫纲,半翅目,黾蝽科)线粒体全基因组注释(英文)

THE COMPLETE MITOCHONDRIAL GENOME OF AQUARIUS PALUDUM(FABRICIUS)(INSECTA,HEMIPTERA,GERRIDAE)

昆虫纲半翅目异翅亚目黾蝽科圆臀大黾蝽 Aquarius paludum ( Fabricius,1794) 已成为生物学研究的理想生物材料之一,为更全面了解其分子生物学特征,本研究测定了圆臀大黾蝽 Aquarius paludum线粒体基因组全序列。该基因组全长15 380 bp,为双链环状 D N A 分子,包含 13 个蛋白编码基因、22 个 tRNA 基因、2 个 rRNA 基因及一个控制区。其基因排序与已报道的其它大部分异翅亚目类群排列方式相同。该基因组基因排列紧密,共观察到 64 bp 基因间隔 ( 除控制区 781 bp 外) 与 33 bp 基因重叠。全基因组 AT 含量为75. 7 % ,而控制区 AT 含量仅为 66. 2 % ,密码子使用也显示出 AT 使用偏好。13 个蛋白编码基因中,除 COⅠ、ND5 使用 TTG 作为起始密码子外,其余使用 ATV。此外,7 个蛋白编码基因使用常规三联终止密码子 TAA,TAG 作为终止密码子,其余以 T 作为终止密码子,下游为同链编码的tRN A 基因。在 tRN A-Ser ( G C T ) 二级结构中,D HU 臂缺失,未形成典型的三叶草结构。

Aquarius paludum（Fabricius,1794）（Insecta,Hemiptera,Gerridae） has become an ideal organism for studies in many biological areas.For an attempt to improve our molecular knowledge about it,the complete mitochondrial genome was sequenced in this study.The genome is a circular molecule with 15 380 bp and consists of 13 protein-coding genes,22 tRNA genes,2 ribosomal RNA genes and control region,which share the same arrangement with most of heteropterans that have reported.The 37 genes are closely assembled one after the other,leaving a total of only 64 bp（excluding control region,781 bp） in intergenic spacers and 33 bp overlapped between several gene pairs have been observed.The nucleotide composition bias towards adenine and thymine with a content of A＋T of whole genome is 75.7 %,which is higher than that of control region（（A＋T）% = 66.2 %）.Furthermore,the codon usage was also showed AT bias.Eleven of the 13 protein-coding genes use the initiation codon ATV while CO1 and ND5 start with TTG.Besides,seven of the thirteen are terminated with the common triplet stop codon TAA,TAG and the remainders are stopped with a single T adjacent to a downstream tRNA gene.Due to its absence of DHU arm,tRNA-Ser（GCT） could not be folded into typical secondary structure.