玉米辐射诱变系表型及SSR遗传差异研究

Research on Genetic Difference of Maize Mutants by Irradiation Based on Phenotypic and SSR

以玉米自交系R08和48-2及其9个和13个辐射诱变系为试验材料,利用表型性状与SSR标记研究各诱变系与相应基础材料间的遗传差异。结果表明,诱变系在吐丝期、株高、株型、粒色及单株产量等性状和分子水平上均发生了不同程度的变异,其中,R08诱变系12、17和48-2诱变系23、27、28、29、31表型变异较大,R08诱变系16和48-2诱变系22、26、33表型变异则相对较小;R08和48-2与相应诱变系位点多态性信息量(PIC)分别在0.18～0.70和0.34～0.83之间,平均分别为0.46和0.61;诱变系与R08和48-2的遗传相似系数分别在0.474～0.842和0.463～0.782之间,平均分别为0.712和0.645。诱变系与基础材料间存在真实的遗传差异。

The genetic difference between mutants and corresponding basic materials was studied based on phenotypic characters and SSR markers, in which maize inbred lines R08 and its nine mutants, 48-2 and its thirteen mutants were selected as materials. The results showed that different degree variation of days to silking, plant height, plant type, seed color, yield per plant, etc. as the molecular level was detected in mutants. Among them, phenotypic variation of R08 mutants 12, 17 and 48-2 mutants 23, 27, 28, 29 and 31 were larger, oppositely, R08 mutants 16 and 48-2 mutants 22, 26, 33 were relatively smaller. The amplitude of polymorphism information content （PIC） between R08, 48-2 and their corresponding mutants were 0.18 - 0.70 and 0.34 - 0.83, and the average values of them were 0.46 and 0.61 respectively. The genetic similarity coefficient between R08 and its mutants ranged from 0.474 to 0.842 with an average of 0.712, 48-2 and its mutants varied from 0.463 to 0.782 with an average of 0.645. Experiments proved that genetic difference between mutants and basic material was true.