细胞色素b_5-蛋白质相互作用研究

Cytochrome b_5-Protein Interactions

蛋白质-蛋白质相互作用在生命过程中发挥至关重要的作用,特别是血红素类蛋白。细胞色素b5(Cyt b5)是血红素蛋白的一个典型代表,在生物体内通过多种蛋白质-蛋白质相互作用来执行其生物功能。目前所揭示的与Cyt b5相关的蛋白质相互作用包括:细胞色素b5-细胞色素b5还原酶,细胞色素b5-细胞色素P450,细胞色素b5-细胞色素c,细胞色素b5-肌红蛋白或血红蛋白,细胞色素b5-融合蛋白(谷胱甘肽S-转移酶GST和绿色荧光蛋白GFP)和细胞色素b5-转运蛋白(蔗糖转运蛋白SUT1和山梨醇转运蛋白SOT6)等。同一蛋白能与众多不同蛋白相互作用的事实,使我们认识到某些特定蛋白的生物学重要性。另一方面,研究同一蛋白与不同蛋白质间的相互作用将会进一步加深我们对蛋白质结构与功能关系的理解,以及指导新颖蛋白的理性设计与最终应用。

Protein-protein interactions play crucial roles in biological processes, especially for heme proteins. Cytochrome b5 （Cyt b5 ） is a typical heme protein, which functions in vivo through various protein-protein interactions. As reviewed herein, the currently revealed interactions associated with Cyt b5 include Cyt b5-Cyt b5 reductase, Cyt b5-Cyt P450, Cyt b5-Cyt c, Cyt b5-myoglobin or hemoglobin, Cyt bs-fusion proteins （glutathione S-transferase, GST, and green fluorescence protein, GFP） , and Cyt b5-tansporters （ sucrose transporter, SUT1, and sorbital transporter, SOT6） , etc. The fact that a single protein can interact with various proteins makes us realize the biological importance of some particular proteins. On the other hand, the study of a single protein interacting with various proteins will further our understanding of protein structure-function relationship, as well as guide rational design of novel proteins for ultimate applications.