单次高剂量率辐射和持续低剂量率辐射对外周血淋巴细胞培养的对比研究

Evaluation of the Biological Effects of High-dose-rate and Continued Low-dose-rate Irradiation for Peripheral Blood Lymphocytes:A Comparative Study

目的:通过外周血淋巴细胞培养,对比研究单次高剂量率外放射和持续低剂量率内放射的辐射生物效应。方法:应用直线加速器对外周血淋巴细胞分别进行1、2、4、8、16、32Gy的单次辐射;应用P-32胶体0.01、0.1、0.5、2.7MBq,分别进行持续的外周血淋巴细胞辐射,培养3d后进行制片染色,对比分析研究细胞核的形态大小和染色体形成率。结果:单次1、2、4、8、16、32Gy的外放射后,外周血淋巴细胞染色体形成率分别为4.7%、5.5%、3.6%、1.7%、0.1%、0.02%;P-32胶体0.01、0.1、0.5、2.7MBq,连续3d的内辐射后,染色体形成率分别为3.3%、2.6%、1.7%、0.004%,未辐射对照组淋巴细胞形成率为3.5%。结论:单次小剂量(1、2、4Gy)辐射对外周血淋巴细胞培养可能有促进作用,单次高剂量(8、16、32Gy)辐射有明显抑制作用,高活度的P-32胶体(0.5、2.7MBq)对外周血淋巴细胞生长有明显抑制作用,对比认为,P-32胶体0.5MBq持续3d辐射的生物效应近似单次8Gy的外放射生物效应。

Objective To evaluate the biological effects of the high-dose-rate and continued low-dose-rate irradiation for peripher- al blood lymphocytes. Mothods The peripheral blood lymphocytes were given 1,2,4,8,16,32 Gy radiation by linear accelerator and were irradiated by 0.01,0. 1,0.5 and 2.7 MBq P-32 colloid respectively. The sizes of lymphoeytes＇ nucleus were analysis and the rates of chromosome formation were calculation. Flostllts After 1,2,4,8,16,32 Gy radiation, the chromosome formation rates were 4.7%, 5.5%, 3.6%, 1.7%, 0.1%, 0.02%. After irradiation of 0.01,0.1,0.5 and 2.7 MBq P-32, the chromosome formation rates were 3.3% , 2.6% , 1.7% , 0. 004% indivually. The lymphocyte formation rate of control group without radiation was 3.5%. Gonclusion A small dose （ 1,2,4 Gy） single radiation could promote the growth of peripheral blood lymphoeytes, and high dose （8, 16,32 Gy） could inhibit the growth. Also, high activity P-32 colloid （0.5,2.7 MBq） inhibit the growth of lymphocytes. In addition, the biological effects of P-32 colloidal 0.5 MBq last three days were similar single radiation of 8Gy.