血清GP73在肝病患者中的表达水平

The Expression Level of the Serum GP73 in Diagnosis of Liver Disease

目的:探讨GP73的检测在肝脏疾病临床诊断中的应用价值。方法:收集295例各类肝脏疾病患者(213例病毒性肝炎、26例酒精性肝炎、23例自身免疫性肝炎和33例非酒精性脂肪肝)及33例健康对照组血清,采用酶联免疫分析和化学发光分析分别对血清GP73进行定量检测,并对治疗前后进行分析比较;应用生化分析仪检测ALT、ALP、AST、GGT、CHE、ALB、TBIL、AFP的含量,运用荧光定量PCR方法检测HBV DNA拷贝,分析不同肝病患者血清GP73含量与其他指标的关系。结果:治疗前病毒性肝炎组、酒精性肝炎组、自身免疫性肝炎组其GP73含量的中位数均>健康对照组(U分别为240.4、24.0和27.0),非酒精性脂肪肝组与健康对照组无统计学意义(U=157.5,P>0.05);病毒性肝炎组与酒精性肝炎组、自身免疫性肝炎组比较有统计学意义(U分别为760.5和1299.0,P<0.05);病毒性肝炎组、自身免疫性肝炎组治疗前后均有统计学意义(U分别为67.2、99.4,P<0.01),酒精性肝炎组治疗前后无统计学意义(U为1322,P>0.05)。血清GP73与CHE、ALB呈负相关(r分别为-0.454,-0.469),与GGT、TBIL呈正相关(r分别为0.34和0.39)。结论:血清GP73在病毒性肝炎、酒精性肝炎和自身免疫性肝炎中表达上调,可作为病毒性肝炎和自身免疫性肝炎患者治疗疗效的观察指标之一。

Objective To explore the applicative value of the serum GP73 test in clinical diagnosis of liver disease. Methods 295 patients sera（213 cases of viral hepatitis, 26 patients with alcoholic hepatitis, 23 cases of autoimmune hepatitis and 33 cases of nonalcoholic fatty liver disease） and 33 healthy controls were collected. The level of GP73 in serum was measured by using ELISA then compared beth before and after treatment, AFP was measured by chemiluminometry method . The levels of ALT, ALP, AST, GGT, CHE, ALB, TBIL,AFP in serum were measured by biochemical analyzer. The HBV DNA was detected by Fluorescent Quantitative PCR （FQ-PCR）. The relevancies between serum GP73 and other markers were analyzed. Results The level of sera GP73 before treatment in viral hepatitis, alcoholic hepatitis and autoimmune hepatitis patients was higher than that of controls （ U were240.4,24.0 and 27.0, espectively ,P 〈0.05）;while it is no significance between nonalcoholic fatty liver disease and controls （U = 157.5 ,P 〉 0.05）, which of sera in viral hepatitis group, autoimmune hepatitis group between before and after treatment were statistically signifi- cant （U were 57.2 and 99.4,respectively ,P 〈0.01 ） ,while it is no significance in alcoholic hepatitis between before and after treat- ment （U = 1322, P 〉 0.05） ; Serum GP73 levels were negative correlated with the serum levels of CHE and ALB （r were 4）. 454 and - 0.469, repectively ） and were positive correlated with the serum levels of GGT and TBIL （ r were 0. 342 and 0.392, separately）. Conclu- tion The expression level of serum GP73 in viral hepatitis, alcoholic hepatitis and autoimmune hepatitis was risen, it can be used as curative effect observation index in viral hepatitis and autoimmune hepatitis patients.