人肺癌PG细胞培养上清液对PBMC凋亡的影响

Effects of PG cell culture supernatant on apoptosis of peripheral blood mononuclear cell

目的:探讨PG肿瘤上清对外周血单个核细胞(Peripheral blood mononuclear cell,PBMC)的增殖抑制及诱导凋亡方面的影响,并探讨了PG细胞影响PBMC凋亡的作用机制。方法:在PBMC培养体系中分别加入不同条件的PG培养上清。在24、72小时收集PBMC,采用流式细胞术测定其对PBMC细胞周期、凋亡率的影响,另外,通过免疫印迹法、流式细胞术分别测定了Bcl-2、Fas蛋白表达的情况来观察PBMC凋亡相关蛋白的变化。结果:PG肿瘤上清没有抑制PBMC进入细胞周期,在PG上清的作用下,PBMC凋亡率显著增加,并且发现PG上清能够调节PBMC内凋亡相关蛋白Bcl-2、Fas的表达量。结论:PG上清能够调节PBMC凋亡相关蛋白量,进而诱导PBMC凋亡,这可能是导致肿瘤微环境中PBMC数量低下的主要原因。

Objective：To analyze the influence of PG cells culture supernatant on proliferation inhibition and apoptosis of PBMC and investigate the probable mechanism.Methods：Different supernatants from PG cells were added into the culture system of PBMC.The PBMC were obtained after 24,72 h of treatment,and the PBMC cell cycle,apoptosis rate were detected using flow cytometry.In addition,the change of apoptosis related proteins Bcl-2,Fas were detected by flow cytometry and Western blot,respectively.Results：The addition of the supernatant from PG cell culture system did not inhibit PBMC into cell cycle but the apoptosis rate of PBMC showed significantly increased.At the same time,we found that supernatant of PG cell can regulate the expression of apoptosis related proteins Bcl-2 and Fas.Conclusion：PG secretions were able to reduce the number of PBMC may be related to the supernatant of PG cell can regulate the expression of apoptosis related proteins and induce the PBMC apoptosis.