国产环丙氟哌酸体外抗菌活性研究

IN VITRO ANTIBACTERIAL ACTIVITY OF HOME-MADE CIPROFLOXACIN

对国内两个研制单位提供的喹喏酮类抗菌药新品种环氟丙哌酸(cipr-ofloxacin) 进行体外抗菌作用评价,并与其它喹喏酮类抗菌药进行比较,用临床常用的氨苄青霉素,头孢唑啉与庆大霉素作为对照。结果表明两个研制单位提供的环丙氟哌酸体外抗菌活性无差异。国产环丙氟哌酸抗菌谱广,抗菌活性强,在现有喹喏酮系列品种中抗菌活性最强。本品对革兰氏阴性肠道杆菌MIC_(50)≤0.25mg/l,MIC_(90)≤0.5mg/l,1mg/l的本品能抑制96%的绿脓杆菌。对于金黄色葡萄球菌本品的MIC_(50)、MIC_(90)分别为0.5,1mg/l,与甲氟哌酸相似,优于头孢唑啉。与国外文献报道结果一致。本品体外抗菌作用受细菌接种量影响不明显。本研究还表明环丙氟哌酸为一杀菌剂,对临床常见致病菌如:大肠杆菌、克雷伯肺炎杆菌、变形杆菌、绿脓杆菌、阴沟肠杆菌及金黄色葡萄球菌均有良好的杀菌作用。

A comparison was made of the in vitro activity of two home-made ciprofloxacins, which provided by two different Institute of Developing New Antibiotics, with other quinolones, beta-lactams and amin-oglycodides (total 11 antibiotics) against 1164 clinical isolates of 19 species,including E. coli, Klebsiellas, Ps. aureginosa, Protues, Enter. cloacae,K. oxytoca, Samonellas, Shigellas, S. aureus, S. epidermidis, etc. Minimalinhibitory concentrations (MICs) were dertermined by agar dilution methodusing multipoint inoculator (Denley Instruments) and a final inoculum of approximalely 10 CFU/ml plates were incubated for 18 hours at 37℃. TheMIC was defined as the lowest antibiotic concentration completely inhibitinggrowth. The bactericidal activity of ciprofloxacins was tested using MBCand a killing curve method. The inoculum effect on ciprofloxacin's activitywas also tested in this study. The results showed that two ciprofloxacinshad no difference in their in vitro antibacterial activity. The findingsapper that home-made ciprofloxacins were with broad antibacterial spectrum and had the strongest antibacterial activity in quinolones. The invitro activity of the compounds against bacterial enteric pathogens (MIC50:<0.25mg/l: MIC90: <0. 5mg/l) was more active than that of cefotaxime,one of the third generation cephalosporins. 1mg/l of the compounds was themost effective inhibiting the growth 96% of Ps. aeruginosa isolates, theactivity of the compounds against Ps. aeruginosa was more effective thanthat of piperacillin and cefoperazone. Against Staph aureus (MIC50, MIC90values were 0.5, 1 mg/l, respectively), the activity of ciprofloxacins wassimilar to that of pefloxacin and stronger than that of penicillins andcefazolin. All findings showed in the report are consistent with those repo-rted by other authors. The new drug's activity was not influenced by thebacterial inoculum. The home-made ciprofloxacins also appered fine bacte-ricidal activity against clinical common pathogens such as E.coli, K1.pneumonia, Proteus Ps. aeruginosa, Enterobacter and Staph aureus.