菝葜高效液相色谱指纹图谱的建立

Finger Print of Smilax china L.by High Performance Liquid Chromatography

目的建立菝葜提取物的高效液相色谱(HPLC)指纹图谱测定方法,并同时对其中落新妇苷和黄杞苷的含量进行测定。方法采用DIKMA Diamonsil C18柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸溶液梯度洗脱,体积流量1.0 mL.min-1,柱温30℃,检测波长303 nm。结果落新妇苷和黄杞苷分别在0.08～20.52μg和0.08～20.28μg范围内线性关系良好,r分别为0.997和0.998。建立10个不同产地样品的HPLC指纹图谱。结论该方法准确、稳定、可靠,可用于菝葜的质量研究和评价。

Objective To establish a finger print and quantitative determination of astilbin（A） and engelitin（E） simultaneously for Smilax china L.of different batches.Methods The samples were separated on a DIKMA Diamonsil C18 column with gradient elution of methanol and 0.1% phosphoric acid at a flow rate of 1.0 mL·min-1 and detected wavelength at 303 nm.Results The linear ranges of A and E were 0.08–20.52 μg（r=0.997） and 0.08–20.28 μg（r=0.998）,respectively.The HPLC finger print of Smilax china L.of ten different places was established.Conclusion The method can be used for quality control of Smilax china L.