复方当归微乳中桂皮醛和藁苯内酯的含量测定

Determination of Ligustilide and Cinnamylaldehyde in Compound Microemulsion of Radix Angelicae Sinensis(Danggui)

目的建立高效液相色谱(HPLC)法测定复方当归微乳中主成分藁苯内酯和桂皮醛的含量,为其质量标准的建立提供依据。方法色谱柱为Inertsil ODS-3柱(4.6 mm×150 mm,5μm),流动相为乙腈-水(55∶45),流速1.0 mL.min-1,检测波长:285 nm,柱温30℃。结果桂皮醛在0.484 5～38.750 0μg.mL-1浓度范围内呈良好的线性关系,回归方程为Y=27.060X-1.586(r=0.999 8),加样回收率为99.60%,日内和日间精密度RSD低于1.25%;藁本内酯在0.531 0～106.100 0μg.mL-1范围内线性关系良好,回归方程为Y=13.930X+1.344(r=0.999 8),加样回收率为98.92%,日内和日间精密度RSD低于1.25%。结论建立的HPLC法专属性强、灵敏度高,可用于复方当归微乳的质量控制。

Objective To establish a method to determine content of ligustilide and cinnamylaldehyde in compound microemulsion of Radix Angelicae Sinensis（Danggui）（CMR）.Methods High performance liquid chromatograph was used for detection.The column of Inertsil ODS-3（4.6 mm×150 mm,5 μm） was used and the detection wavelength was 286 nm.The mobile phase was Acetonitrile-water（55：45） at a flow rate of 1.0 mL·min-1 and the temperature of column was set at 30 ℃.Results The calibration curve of Cinnamaldehyde was linear in the range of 0.484 5-38.75 μg·mL-1.The regression equation was Y=27.06X-1.586（r=0.999 8）.The recovery rate was 99.60%.The intra-day and inter-day precision was good with the RSD of 1.251% or below.The calibration curve of ligustilide was liner in the range of 0.531 0-106.1 μg·mL-1.The regression equation was Y=13.93X＋1.344（r=0.999 8）.The recovery rate was 98.94%.The intra-day and inter-day precision was good with the RSD of 1.25% or below.Conclusion This method is convenient,rapid and accurate.It can be used for quality control of CRM.