摘要
该文从杨树 -光肩星天牛的协同进化关系出发 ,应用同工酶分析法对毛白杨、I 69杨和小美旱杨进行了生态抗虫性的研究 .根据聚丙烯酰胺凝胶电泳方法对对照树的酶谱分析表明 ,3种杨树的过氧化物酶 (POD)、多酚氧化酶 (PPO)和酯酶 (EST)各有其特有的酶谱 ,毛白杨POD具有特异性条带 ;根据比色方法对酶活性分析得毛白杨POD含量最高 ,活性最强 ,通过方差分析和多重比较得 ,毛白杨与其它两种杨树之间的POD酶差异极显著 .随后又对危害树进行了分析 ,测得毛白杨POD增长率最大 (为 17% ) .由此作者认为POD ,PPO ,EST和蛋白质 (PRO)都与抗虫性有一定的关系 ,其中POD与抗虫性的关系最大 ,可能由抗虫基因所控制 .
An isozyme survey on three poplars was undertaken in view of coevolution to investigate the different resistance to Anoplophora glabripennis . The three species were Populus tomentosa Carr. (high resistance), P. deltoides Bartr. cv.`Lux' (I\|69/55) (resistance) and P. simonii × P. nigra var. italica (susceptible ).Three enzymes and protein were examined using Polyarylamide Gel Electrophoresis (PAGE). Clear consistent banding patterns were obtained for EST, POD, PPO and PRO. POD has a special band. Variation analysis showed that three poplars differed evidently in POD . Moreover, surveys showed that POD,PPO,EST and PRO increased differently in damaged trees, of which P. tomentosa increased by 17% in POD . For the above reasons the authors inferred that three enzymes and protein were relative to the resistance, but the most important was POD .
出处
《北京林业大学学报》
EI
CAS
CSCD
北大核心
2000年第1期91-94,共4页
Journal of Beijing Forestry University
基金
国家自然科学基金资助项目!( 3 95 70 12 1)
