一株脱硫菌的鉴定及其对H_2S的降解能力

Identification of a Desulfurizing Strain and Its H_2S Degrading Capacity

取污水处理厂有生物活性的污泥,经富集培养、分离纯化得到1株脱硫活性较高的菌株CTD843-T-3,将其载入生物滴滤塔中对H2S进行连续脱硫反应。当最佳反应温度为30℃、pH为6.0、H2S进口浓度为500～3 000mg·m-3时,菌株对H2S的去除率可保持在92%以上,H2S最高负荷率可达6.5 g·m-3·h-1。通过形态生理特征和16S rDNA序列分析对CTD843-T-3进行鉴定,结果表明该菌株为椭球状,大小为0.4～0.6μm,为革兰阴性菌,属假单胞菌属(Pseudomonas sp.)。

An active desulfurizing strain of bacteria CTD843 - T - 3 was isolated from bio-active sludge collected in a wastewater treatment plant and loaded into a bio-trickling reactor for consecutive desulfurization of H2S. When the reaction temperature was adjusted to 30 ℃, pH to 6.0, and the inlet concentration of H2S to 500 -3 000 g·m^-3, the H2S removal rate of the strain could be kept over 92% , and the maximum H2S loading rate could reach 6. 5 g·m^-3·h^-1 Through the morphological, biochemical ＆ physiological identification, and 16S rRNA sequence analysis, the strain was found to be ellipse-shaped, 0.4 -0.6 μm in size, gram-negative, and a member of Pseudomonas.