三种结核分枝杆菌重组蛋白抗原血清学诊断价值的研究

Research on serodiagnostic value of three recombined antigens from M.tuberculosis

目的研究重组ESTA6(rESTA6)、CFP10(rCFP10)、CFP10-ESTA6(rCFP10-ESTA6)三种结核分枝杆菌重组抗原在结核病血清学诊断中的应用价值。方法检测230例肺结核、70例非结核疾病及200例健康血清标本中抗结核抗体,应用SPSS18.0对检测结果进行方差分析。结果方差分析表明,rCFP10、rESAT6、rCFP10-ESAT6三种抗原对肺结核、非结核疾病及健康人血清标本的检测结果有统计学意义(P<0.05),三种抗原检测肺结核病患者血清标本的敏感性分别为31.3%、25.2%、34.8%,特异性分别为94.8%、97.8%、95.6%。rCFP10和rESAT6联合检测敏感性及特异性分别为40.0%和93.0%;rCFP10和rESAT6联合检测的92例阳性标本中,有23例rCFP10-ESAT6检测呈阴性;rCFP10-ESAT6检测的80例阳性标本中,有26例rCFP10和rESAT6联合检测呈阴性。结论 rCFP10-ESTA6的结构与rCFP10和rESTA6的单体的结构可能不完全相同,rCFP10-ESTA6抗原不能完全代替rESTA6、rCFP10两种抗原进行结核病的血清学实验室诊断。

Objective To evaluate the value of tESTA6, rCFP10 and rCFP10-ESTA6 protein from M. tuberculosis on the serodiagnosis of tuberculosis（TB）. Methods The recombined protein was expressed and purified by biotechnology. 230 cases of pulmonary tuberculosis patients ,70 cases of non tuberculous patients and 200 cases of healthy subjects sera were detected the antibodies against the antigens by ELISA. The data obtained were analyzed for ANOVA using SPSS 18.0 software package. Results The One-Way ANOVA showed that the level of anti-TB antibodies had significant difference （P 〈 0. 05 ）between the three groups＇serum. The sensitivities of rESTA6, rCFP10 and rCFP10-ESTA6 were 31.3% , 25.2% and 34. 8% , the specificities were 94. 8% , 97.8% and 96. 0% , respectively. The sensitivities and specificities of combination antigens （ rCFP10 and rESAT6 ） were 40. 0% and 93. 0%. In 92 combination antigens-IgG positive case there were 23 rCFP10-ESAT6-IgG negative ones, and 26 cases combination antigens-IgG negative in 80 CFP10-ESAT6-IgG positive. Conclusions The structure of rCFP10-ESTA6 is not completely as same as the rESTA6 and rCFP10, hence it can＇ t replace the rCFP10 and tESTA6 in the serodiagnosis of TB.