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新型T+1断裂蛋白质内含子的构建

Construction of Functional Split Inteins in front of a Thr Residue
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摘要 首次从天然C端外显子为苏氨酸(Thr)的标准蛋白质内含子(TerSnf2)构建出两类T+1型断裂蛋白质内含子:Snf-S0和Snf-S1,通过蛋白印迹法测定Snf-S0和Snf-S1介导的蛋白质反式剪接效率分别达到53.5%和33.3%;当这两类断裂蛋白质内含子C端外显子分别为半胱氨酸(Cys)和丝氨酸(Ser)时均具有剪接活性.所获的两类具有剪接活性的T+1型断裂蛋白质内含子具备一定的插入位点通用性,这一特点为蛋白质反式剪接技术在蛋白质工程领域广泛应用提供了新的契机. The study is about construction of functional split inteins in front of a Thr residue（Snf-S0 and Snf-S1） from natural canonical intein. Their protein trans-splicing efficiency can reach 53.5 % and 33.3 % respectively. They still have splicing activity in front of a Cys or Set. This study confirms that two kinds of T ＋ 1 type of split intein have a ability of insertional locus of generality. The protein trans-splicing provides more opportunities for wide application in protein engineering technology.
作者 王瑾 林瑛 郑言成 孟清
机构地区 东华大学生物科学与技术研究所
出处 《东华大学学报（自然科学版）》 CAS CSCD 北大核心 2012年第1期50-54,共5页 Journal of Donghua University(Natural Science)
基金 国家"八六三"高技术研究发展计划资助项目(2006AA03Z451) 国家自然科学基金资助项目(30800186) 教育部高等学校博士学科点专项科学基金(新教师基金)资助项目(200802551026)
关键词 断裂蛋白质内含子 蛋白质反式剪接 TER Snf2 split intein protein trans-splicing Ter Snf2
分类号 Q75 [生物学—分子生物学]
  • 相关文献

参考文献17

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二级参考文献14

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东华大学学报（自然科学版）
2012年 第1期

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