姜黄素对急性肺栓塞大鼠氧化损伤及血红素加氧酶-1表达的影响

Effect of curcumin on oxidative injury induced by acute pulmonary embolism in rats and its influence on the expression of heme oxygenase-1

目的观察姜黄素对急性肺栓塞大鼠氧化损伤的作用及对血红素加氧酶-1(HO-1)表达的影响。方法大鼠分为假手术组、模型组、姜黄素低、高剂量(50 mg/kg、150 mg/kg)组。制备急性自体血栓肺栓塞模型。进行血气分析。检测肺组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性、丙二醛(MDA)含量;HE染色观察肺组织病理改变;RT-PCR方法和Western blot法检测肺组织HO-1的表达。结果与模型组比较,姜黄素低、高剂量组可不同程度的升高PO2、PCO2水平,升高SOD、GSH-Px、CAT活性,降低MDA含量,减轻肺组织的氧化病理损伤。姜黄素低、高剂量组均能显著升高肺组织HO-1表达。结论姜黄素对急性肺栓塞大鼠有抗氧化损伤作用,并能上调肺组织HO-1表达。

Objective To investigate the effect of curcumin on oxidative injury induced by autoblood thrombus pulmonary thromboembolism （ PTE ） in rats and its influence on the expression of heine oxygenase-1 （ HO-1 ）. Methods The rats were divided into sham - operation group, model group,low-dose curcumin group and high-dose cureumin group （50 mg/kg, 150 mg/kg,respectively）. The animal models with acute autoblood thrombus PTE were made in rats. The blood gas analysis was carried out. The contents of superoxide dismutase （SOD）, glutathione peroxidase （ GSH-Px）, malondialdehyde （ MDA ） and the activity of hydrogen peroxidase （AT） in pulmonary tissue were detected;the pathological changes of pulmonary tissue were observed by HE staining;the expression of HO-1 was detected by RT-PCR and Western blot. Results As compared with those in model group, the levels of PO2, PCO2, SOD, GSH-Px, CAT and the expression of HO-1 were significantly increased in low-dose curcumin group and high-dose curcumin group, however, the content of MDA was significantly decreased in the two groups. The oxidative pathological lesions of pulmonary tissue were alleviated in cureum groups. Conclusion The curcumin can reduce oxidative injury induced by acute PTE in rats and can up-regulate the expression of HO-1.