甲磺酸酚妥拉明抗心律失常的初步研究

The Primary Research of the Mechanism of anti-arrhythmia by phentolamine mesylate

目的:通过观察甲磺酸酚妥拉明(PM)对正常大鼠心室肌细胞钠电流(INa)的影响,以探讨其抗心律失常的作用机制。方法:采用膜片钳全细胞记录技术记录了PM对单个大鼠心室肌细胞钠电流的影响。结果:动物实验表明PM降低正常心室肌细胞INa电流密度峰值,钠离子通道失活加速,失活后再恢复时间延迟。结论:PM对心室肌细胞0相动作电位快钠电流有抑制作用,能使快速性室性心律失常的发生率降低。

Objective：Through observation the effect of phentolamine mesylate （PM） on sodium current （INa） in rat ventricular myocytes ,in order to explore its antiarrhythmic effect mechanism. Methods：the whole cell patch clamp technique was used to re- cord the effect of PM on the sodium currents in single rat ventricular myocytes. Results ： animal experiments show that PM reduces INa current density peak in normal ventricular muscle cells, the inactivation of sodium channel was accelerated, recovery time was de- layed after inactivation. Conclusion： PM could inhibit the 0 phase of action potential fast sodium current inhibition in ventricular myocytes,and decrease the incidence of ventricular arrhythmia.