屋尘螨抗原致敏大鼠支气管上皮细胞白介素6、白介素8和转化生长因子β1的表达

Derpl induce interleukin-6, interleukin-8, transforming grouth factor-p1 express and release from cultured rat bronchial epithelial cell

目的探讨屋尘螨抗原（Derpl）对大鼠原代支气管上皮细胞白介素6（IL-6）、IL-8和转化生长因子β1（TGF—β1）表达的影响。方法将实验随机分成正常对照组和实验组，实验组中细胞分别暴露于3个不同的Derpl浓度（1、5、10mg，／L）及3个不同的培养时间点（4、8和24h）。然后用逆转录-聚合酶链式反应（RT—PCR）及酶联免疫吸附试验（ELISA）分别检测细胞及细胞上清液中IL-6、IL-8和TGF-β1的表达及含量。结果与对照组相比，实验组各指标有明显变化。Derpl刺激4h，IL-6和IL-8表达开始升高，直至8～24h呈高水平。而在Derpl浓度≥5mg／L条件下，IL-6和IL-8表达最明显，与正常对照组相比差异有统计学意义（P〈0．05）。而TGF—β1则随着时间和浓度的增加，其表达水平呈逐渐上升趋势，各时间点的表达差异均有统计学意义（P〈O．05）。各炎症指标（IL-6，IL-8，TGF-β1）ELISA结果与PCR趋势基本一致。结论Derpl刺激气道上皮细胞，引起支气管上皮细胞免疫反应，释放一系列炎症因子如IL-6、IL-8和TGF-β1，从而参与哮喘气道炎症反应及哮喘的气道重塑。

Objective To investigate the effects of exposure to increasing concentration of Derpl on the expressions of IL-6,IL-8,TGF-β1 in primary rat bronchial epithelial ceils. Methods Ceils were divided into two groups, control groups and experimental groups. In the experimental groups ceils were cultured with 3 different concentrations of derpl （1,5,10 mg/L） for 3 different time （4,8,24 h）, then detected mRNA expression and cytokine release by using RT-PCR and ELISA. Results There was a obvious change in the level of cytokine production compared with the control groups. The levels of IL-6 and IL-8 began to ris＇e at 4 h, and reach to high expression at the 8-24 h. The maximum concentrations were detected Cytokine release was different. IL 6, IL-8 was at concentrations of ≥5 mg/L （ P〈0.05）. But the release of TGF-β1 was Positively related to exposure time and Derpl concentration （ P 〈0.05）. The release of IL-6,IL 8 and TGF-β1 level as well as an increase in the expression of IL 6,IL-8 and TGF-β1 mRNA manifested evidently. Conclusions The Derpl allergen can stimulate the release of inflammatory cytokines from the rat bronehia epithelial ceils, such as （IL-6, IL-8, and TGF-β ）. It is suggested that HDM-induced cytokines may play an airway inflammation and remodeling of allergic asthma.