天麻素对血小板衍生生长因子-BB诱导大鼠血管平滑肌细胞迁移的影响

Effect of gastrodin on rat vascular smooth muscle cell migration induced by platelet-derived growth factor-BB

目的探讨天麻素对血小板衍生生长因子一BB（platelet—derivedgrowthfactor—BB，PDGF—BB）诱导血管平滑肌细胞（vascularsmoothmusclecell，VSMC）迁移的影响及其可能机制。方法酶消化法获取大鼠主动脉VSMC并传代纯化。免疫荧光染色法对VSMC标记蛋白进行鉴定。建立PDGF—BB诱导细胞迁移模型。Transwell小室法评价天麻素对PDGF—BB诱导VSMC迁移的影响。蛋白质印迹法检测c—JunN末端激酶（c-junN—terminalkinase，JNK）磷酸化水平。结果原代培养的VSMC纯度达99％以上。PDGF—BB组VSMC迁移数量为（85．2±3．486）个觎野，显著多于对照组的（42．5±1．927）个/视野（t=9．981，P〈0．001），而天麻素能使PDGF—BB诱导VSMC迁移数量显著减少为（71．3±1．783）个觎野（t=3．550，P=0．002）。蛋白质印迹分析显示，天麻素能抑制PDGF—BB诱导的YNK磷酸化（0．190±0．015对0．190±0．015；t=14．548，P=0．000）。结论天麻素可抑制PDGF—BB诱导VSMC迁移，其机制可能与抑制JNK信号通路激活有关。

Objective To investigate the effect of gastrodin on rat vascular smooth muscle cell （VSMC） migration induced by platelet-derived growth factor-BB （PDGF-BB） and its possible mechanisms. Methods Enzyme digestion method was used to obtain rat aortic VSMCs and be purified by passage. Immunofluorescence staining was used to identify VSMC marker proteins. A PDGF-BB induced cell migration model was established. Transwell chamber assay was used to evaluate the effect of gastrodin on PDGF-BB induced VSMC migration. Western blots were performed to detect the phosphorylation levels of c-jun N-termi- nal kinase （JNK）. Results The purity of primary cultured VSMC was more than 99%. The VSMC migrated number in the PDGF-BB group was 85.2 ± 3. 486 per field. It was significantly more than 42. 5± 1. 927 per field in the control group （t=9. 981, P〈0. 001）, and gastrodin was enable to make PDGF-BB induced the number of VSMC migration significantly reduce to 71.3 ±1. 783 per filed （t = 3. 550, P = 0. 002）. Western blots analysis showed that gastrodin inhibited PDGF-BB induced JNK phosphorylation （0. 190 ± 0. 015 vs. 0. 190± 0. 015; t = 14. 548, P =0. 000）. Conclusions Gastrodin inhibits PDGF-BB induced VSMC migration, its mechanisms may be associated with the inhibition of the JNK signaling pathway activation.