健康成年人血清肌酐生物参考区间调查验证分析

Investigation and analysis of biological reference intervals of serum creatinine in healthy adults

目的通过测定健康成年人群的血清肌酐值,建立本实验室血清肌酐的生物参考区间。方法采用碱性苦味酸法对498例健康成年人进行血清肌酐测定,并按性别和年龄分组比较。结果男性成年人肌酐值高于女性差异有统计学意义(P<0.05);>60岁肌酐值高于其他成年年龄组,差异有统计学意义(P<0.05),年龄18～60岁健康男性95%可信限的参考区间为67.1～119.1μmol/L;>60岁健康男性95%可信限的参考区间为72.0～127.8μmol/L;年龄18～60岁健康女性95%可信限的参考区间为54.4～88.2μmol/L,>60岁健康女性95%可信限的参考区间为60.3～101.5μmol/L。结论生物参考区间对疾病的预防、诊断、疗效及预后均有重要的指导意义,应依据标本类型、检测方法和检测人群制定适合各实验室的生物参考区间。

Objective To establish the biological reference of serum creatinine in the laboratory by measuring serum creatinine in healthy adults. Methods Measured serum creatinine of 498 cases of healthy adults by alkaline picric acid meth- od,compared the results by gender and age. Results Serum creatinine of male adults was higher than that of female adulta, the difference was statistically significant （P 〈 0.05 ） ; Serum creatinine of over 60 years old was higher than other adult age group, the difference was statistically significant （ P 〈 0.05 ）, 95 % confidence limit of reference interval of 18-60 years old healthy male was 67.1-119.1 μmol/L, 〉 60years old was 72.0-127.81μmol/L, 18-60 years old was 54.4-88.21.μmol/L. 95% confidence limit of reference interval of over 60 years old healthy women was 60.3 - 101.51μmol/L. Conclusion Refer- ence intervals are important for early prevention, diagnosis, therapeutic effect and prognosis of disease. Each clinical laboratory should establish itself different reference intervals.