He-Ne激光处理对拟南芥种子萌发和幼苗生长特性的影响

Effects of He-Ne Laser Irradiation on the Seeds Germination and Seedling Growth of Arabidopsis thaliana

以哥伦比亚生态型(Columbia-0)的野生型拟南芥为材料,利用He-Ne激光生物辐照仪(632.8nm,5mW/mm2)处理拟南芥种子,统计其发芽势和发芽率,并测定根长及叶绿素、可溶性糖、可溶性蛋白和丙二醛(MDA)的含量,以探讨He-Ne激光对拟南芥种子萌发和幼苗生长的影响。结果显示,短时间的He-Ne激光辐照可促进拟南芥的生长发育,且最佳辐照时间为4min,此时的发芽势、发芽率、根长、叶绿素、可溶性糖及可溶性蛋白的含量均达到最大,而丙二醛(MDA)的含量为最低。当辐照时间大于4min时,促进作用逐渐变小,且随着辐照时间的延长而表现出抑制作用。研究表明,适量的He-Ne激光辐照在一定程度上可以促进拟南芥种子的萌发和幼苗生长以及保护其细胞膜免受伤害。

We choose Columbia wild type Arabidopsis thaliana as the experimental material, using He-Ne laser instrument（632.8 nm,5 mW/mm2） to handle the seeds,then gather statistics about germination po- tential,germination percentage, root length, the contents of chlorophyll, soluble sugar, soluble protein and MDA. The results showed that, short period of He-Ne laser radiation could promote the growth of A. thaliana. It has an obvious influence when the time reaches 4 minutes, and the germination potential, ger- mination percentage, root length, the contents of chlorophyll, soluble sugar, soluble protein reach the high- est. However, the content of MDA reach the lowest. When the time is beyond 4 minutes, the promotion in- fluence become less,and then it inhibited with longer radiation time. All of these show that suitable He-Ne laser dosage radiation could promote the seeds germination and seedling growth of A. thaliana and protect the cell membrane from being injured.