多个环境下小麦千粒重QTL定位的稳定性分析

The Stability of QTL Mapping for Thousand Grain Weight in Multiple Ecological Environments

为尽可能多的探寻千粒重的重要遗传位点并验证其在不同生态条件下的稳定性,以142个和尚麦/豫8679的F9:10重组自交系(RIL)及其亲本为试验材料,分析了千粒重在北京(2006、2007)、安徽合肥(2007、2008)、四川成都(2007、2008)和山东泰安(2009、2010)8个环境下的性状表现,并利用已构建的含有170个SSR标记和2个EST标记的遗传图谱对该性状进行了QTL定位分析。结果共检测到35个QTLs,可解释表型变异的4.36%～16.80%;涉及小麦1A、1B、2A、2D、3A、3B、4A、4D、5A、5B、6D和7D染色体,特别是1B、2A、3B染色体上(Xwmc269、Xgwm33、Xwmc419、Xbarc124、Xgwm636、Xgwm359、Xgwm533、Xwmc307、Xwmc418)的QTL可在多个环境下稳定的表达,为千粒重的QTL精细定位和分子标记辅助选择奠定了基础。

To identify QTLs related to thousand-grain weight （TGW） and validate its＇ stability in mul- tiple ecological environments, the F9：10 generation of 142 recombinant inbred lines （RILs） derived from the cross between Yu 8679 （large spike） and Heshangmai （small spike） were planted in eight ecological environments in Beijing （2006 and 2007）, Hefei （2007, 2008）, Chengdu （2007, 2008） and Taian （2009, 2010）. A genetic map comprising 170 SSR and 2 EST markers （Tx23-24 and Tx37-38） was constructed based on the 142 RII.s. According to the genetic map and phenotypic data, quantita- tive trait loci were identified for TGW using the composite interval mapping （CIM） method. A total of 35 QTLs located on chromosomes 1A, 1B, 2A, 2D, 3A, 3B, 4A, 4D, 5A, 5B, 6D, and 7D for TGW were identified over eight environments, which accounted for variations of TGW by 4. 36O//oo 16.80~//00. Especially, three QTI.s on chromosomes 1B （QTgw. nfcri-lB）, 2A （QTgw. nfcri-2A） and 3B （QTgw. nfcri-3B） could be detected in the most environments and could explain high phenotypic variation, the three QTLs may be effective and useful for farther exact location and MAS for high yield breeding.