新型肾脏冷缺血再灌注损伤动物模型的稳定性研究

Stability of a new model of kidney cold ischemia reperfusion injury

目的建立一种新型肾脏冷缺血再灌注损伤动物模型,以简化既往模型的复杂性,适用于更多机构及人员进行肾脏冷缺血再灌注损伤相关研究。方法实验于2010年1～10月在解放军第309医院实验动物中心完成。SD大鼠45只随机分成对照组(n=5)、手术组(n=20)和假手术组(n=20),对照组采用经典的Takada肾脏冷缺血再灌注损伤模型,手术组采用新型肾脏缺血再灌注损伤模型,利用术后需被切除右肾动脉插管进行灌注,省略动脉显微缝合步骤。观察手术组和假手术组大鼠术后6 h、1 d、3 d、5 d肾功能、NF-εB及肾脏的病理变化并与对照组比较。结果手术组和对照组相比,本模型大鼠术后各时间段肌酐、尿素氮和NF-εB差异有统计学意义。两组术后第6 h、1 d、3 d肌酐值比较,P<0.05;术后1 d尿素氮比较,P<0.05;术后1 d、3 d NF-εB比较,P<0.05。可能与省略血管缝合步骤,从而减少大鼠热缺血再灌注损伤时间有关,手术组和对照组病理学检查符合急性肾衰竭改变。结论新型肾脏冷缺血再灌注损伤模型操作更为简单,稳定性强,利于被普通研究人员所掌握,成功率高,是一种研究肾脏冷缺血再灌注损伤的较好模型。

Objective To prepare a stable animal model of rat renal ischemia reperfusion injury and simplify the complexity of model,apply more institution and people to research.Methods The experiment was completed in the center for experimental animal,the 309th hospital of Chinese PLA from January to December in 2010.Forty-five SD rats were randomly divided into control group（n=5）,operation group（n=20）and sham group（n=20）.The control group apply the model of Takada′s renal cold ischemia reperfusion injury,The operation group apply the new model of renal cold ischemia reperfusion injury,utilize the right renal artery to perfusion which need to cut after operation,omit the procedure of vascular suture.The renal function,NF-εB and pathological changes from 6 h,1 d,3 d,5 d after operation of operation group and sham operation group were observed and compared with that of the control group.Results Compare with control group,creatinine,blood ureanitrogen and NF-εB in the model of operation group were lower in some times after transplantation（creatinine in 6 h,1 d,3 d respectively,P〈0.05;blood ureanitrogen in 1 d respectively,P〈0.05;NF-εB in 1 d,3 d respectively,P〈0.05）,which relate to omitting the procedure of vascular suture,and reducing the time of rat renal ischemia reperfusion injury.The pathological changes of the operation group and control group accorded with acute renal failure.Conclusions The preparation for the new model of renal cold ischemia reperfusion injury is simple,stable and good for common researcher and with a high successful rate,so such a model can be used to study renal cold ischemia reperfusion injury.