NKG2A、NKG2D在食管鳞癌不同病理分期的表达及其相关性分析

Expression of NKG2A and NKG2D in different stage of esophageal squamous cell carcinoma and its correlation

目的分析不同病理分期食管鳞癌患者血清中自然杀伤细胞表面抑制性受体A(NKG2A)和自然杀伤细胞表面活化性受体D(NKG2D)表达水平的特点,探讨NKG2A和NKG2D在食管鳞癌不同病理分期的表达意义,并研究NK细胞、NKG2A、NKG2D与病理分期的相关性。方法用流式细胞仪分析92例食管鳞癌患者及50例健康志愿者外周血NK细胞数量及其NKG2A和NKG2D的表达情况。结果与正常对照组相比,食管鳞癌患者外周血NK细胞表达增加[(9.97±4.25)%vs.(16.22±8.91)%,t'=-5.646,P<0.01],NK细胞表面NKG2A的表达水平升高[(35.19±17.73)%vs.(47.35±18.88)%,t=-3.742,P<0.01],NKG2D的表达水平降低[(83.20±3.85)%vs.(80.60±9.09)%,t'=1.925,P=0.019];食管鳞癌患者NKG2A/NKG2D的比值与其病理分期呈正相关性(r=0.333,P<0.01),行线性回归分析得F=7.413,P=0.008,按α=0.05水准,回归方程为:Y(NKG2A/NKG2D的比值)=0.105X(食管鳞癌病理分期)+0.347;R2=0.276。结论食管鳞癌患者机体NK细胞数目增多,NKG2A/NKG2D比值与食管鳞癌病理分期进展呈正相关;降低NKG2A,提高NKG2D的表达水平,能增强NK细胞对肿瘤的杀伤活性,从而为食管鳞癌的免疫治疗开创新的思路。

Objective To analyse the expression levels of NKG2A and NKG2D in different pathological （TNM） stage of esophageal squamous cell carcinoma patients, discuss the meaning and research of correlation between NK cells,NKG2A, NKG2D and pathological stage. Methods Using flow cytometry to analyse the numbers of NK cells and expression of NKG2A and NKG2D in peripheral blood of 92 cases esophageal squamous cell carcinoma patients and 50 healthy volunteers. Results Compared with the normal control group, the expression level of NK cells in peripheral blood of esophageal squamous cell carcinoma patients increased [ （9. 97 ±4. 25 ） % vs. （ 16. 22 ± 8.91 ） %, t＇ = - 5. 646, P 〈 0. 01 ], and the expression level of its surface receptor NKG2A increased [ （35. 19 ± 17. 73）% vs. （47. 35 ± 18.88）% ,t = -3. 742,P 〈0. 01 ] ,but the expression level of NKG2D reduced [ （83. 20±3.85）% vs. （80. 60 ±9.09）% ,t＇ = 1. 925 ,P =0. 019]. It was showed a positive correlation between the ratio of NKG2A/NKG2D and the pathological stage of esophageal squamous cell carcinoma（ r = 0. 333, P 〈 0. 01 ）, Line linear regression analysis （F = 7. 413, P = 0. 008, at ot = 0. 05 level）got regression equation ： Y （NKG2A/NKG2D ratio） = 0. 105X （pathological stage of esophageal squamous cell carcinoma） ＋ 0.347 （R2 = 0.276 ）. Conclusions The number of NK cells in esophageal squamous cell carcinoma increased. NKG2A/NKG2D ratio and the pathological stage progress of esophageal squamous cell carcinoma showed a positive correlation. Reduced NKG2A,increased NKG2D expression levels can enhance killing activity of NK cells, so as to open up new ideas about the esophageal squamous cell carcinoma immune therapy.