紫叶碧桃花色苷提取及其稳定性研究

Study on the Extraction and Stability of Prunus persica Batsch.

研究了花瓣花色苷的提取方法及不同浓度的双氧水、亚硫酸钠、蔗糖、葡萄糖、柠檬酸、Na+、Zn2+、Ca2+、Cu2+溶液及不同pH等对紫叶碧桃花色苷的影响。结果表明:以0.5%HCl和75%乙醇为浸提剂在65℃恒温提取3h,提取效果最好。在影响其稳定性的各因子中,pH对花色苷影响显著,在酸性条件下,花色苷具有良好的热稳定性。金属离子Na+、Zn2+、Ca2+在不同浓度下对花色苷影响不显著,Cu2+较显著。花色苷的还原能力不显著,抗氧化性显著。在自然光和黑暗条件下花色苷吸光度均不显著。蔗糖、葡萄糖、柠檬酸对其颜色与性质的影响均不显著。

The influence on the Prunus persica pigment of the method for extracting flower petal pigment and the different concentration of hydrogen peroxide solution,the sodium sulfite,the sucrose,the glucose,the citric acid,Na＋,Zn2＋,Ca2＋,Cu2＋ solution,different pH and so on were studied.The results showed that the optimum yield of anthocyan could be obtained by 75% ethanol and 0.5% hydrochloride at 65℃ for 3 h.In the different factors that influenced stability of the Prunus persica anthocyan,pH had obvious effect on anthocyanin pigment.In the acid condition,the anthocyan was capable of keeping its good heat resistance.Metallic ions such as Na＋,Cu2＋,Zn2＋,Ca2＋ had no evident effect on the anthocyan except Cu2＋.The anthocyan had a weak reducibility besides apparent antioxidant.In natural light and dark conditions,the absorbency of anthocyan were not significant.The sucrose,the glucose and the citric acid had a weak impact on its colour and character.