摘要
以欧美杨107为试材,采用农杆介导法进行双抗虫基因(Bt Cry1Ac基因+API慈姑蛋白酶抑制剂基因)的遗传转化研究,探讨了影响欧美杨107的遗传转化的不同因素,初步建立了欧美杨107品种高效组织培养再生体系及遗传转化体系。结果表明:经PCR检测和虫试效果,证明了目的基因已经整合到欧美杨107的基因组中,从转基因株系中筛选出生长发育正常并抗虫能力强的优良株系。
Taking Poplar 107 as material,and two kinds of insect genes（BtCry1Ac genes and API genes） genetic transformation study was mediated by the agrobacterium,the impact factors to Poplar 107 during the genetic transformation were discussed,and the more efficient tissue culture regeneration and genetic transformation system for Poplar 107 were established.The results showed that after the PCR detection and worm test results,it proved that the aim gene had been integrated into the Popar 107 genome,and the target was to select the top-quality plants with normal growth and strongly construct insect ability from the transgenic plants.
出处
《北方园艺》
CAS
北大核心
2012年第7期120-122,共3页
Northern Horticulture
基金
国家高技术研究发展计划("863"计划)资助项目(2011AA100201)
关键词
欧美杨107
农杆菌
遗传转化
再生体系
检测
Poplar 107
Agrobacterium tumefaciens
genetic transformation
regeneration system
detection
