生防菌防治黄瓜枯萎病的协同作用研究

The Synergism of Different Biocontrol Agents to Cucumber Fusarium wilt

采用传统方法统计可培养微生物的数量,采用常规方法测定土壤酶活、统计黄瓜形态指标和发病株数,探讨不同生防菌株枯草芽孢杆菌D、放线菌317和木霉菌联合防治黄瓜枯萎病是否具有协同增效作用。结果表明:3株菌株联合优于单一菌株和2株菌株联合,其协同增效作用最佳。与对照相比,3株菌株联合处理根际土壤中细菌、放线菌、真菌数量的接种增率分别为50.57%、110.98%和13.48%,菌群结构开始由真菌型向细菌型过渡;土壤中性磷酸酶、蔗糖酶、过氧化氢酶和脲酶活性增长率分别为244.90%、7.17%、2.90%和31.01%;黄瓜株高、茎粗、叶片数和干物质重量的增长率分别为33.92%、35.48%、34.43%和79.13%;防治效果最佳达52%。与其它处理相比,3株菌株联合处理对根际土壤微生物数量、菌群结构、过氧化氢酶、黄瓜的形态指标和枯萎病的防治效果具有明显的协同增效作用,其在生产中具有较大的应用潜力。

The number of culturable microorganisms were measured by traditional method.Soil enzyme activity,plant phenotypic traits and incidence number were determined by routine analysis methods,to explore the synergism of biocontrol agents bacteria D,actinomycetes 317 and fungi compound to cucumber Fusarium wilt.The results showed that three agents compound was superior to single agent and two agents compound,its synergism was the best.Three agents compound was compared to control;number of bacteria,actinomycetes and fungi could raise 50.57%,110.98% and 13.48%;microflora was becoming fungi type to bacteria type;activity of phosphatase,invertase,catalase and urease could raise 244.90%,7.17%,2.90% and 31.01%;plant height,stem diameter,leaf number and dry matter weight could raise 33.92%,35.48%,34.43% and 79.13%;control effect could raise 52%.Compared to control,three agents compound had clear synergism in number microorganism,microflora,plant phenotypic traits and control effect.The synergism to phosphatase,invertase and urease was not clear,but to catalase was clear.Three agents compound would have great application potential in production.