不同提取方法对桦褐孔菌多糖抗氧化活性的影响

Effects of Different Extraction Methods on Antioxidant Activity of Inonotus obliquus Polysaccharides

[目的]研究不同提取方法对桦褐孔菌多糖抗氧化活性的影响。[方法]采用水煮醇沉法和碱液醇沉法提取粗多糖;采用三氯乙酸法纯化水溶和碱溶粗多糖;采用羟基自由基清除试验和超氧阴离子自由基清除试验进行抗氧化活性对比研究。[结果]水溶粗多糖、碱溶粗多糖、水溶纯多糖、碱溶纯多糖的多糖得率分别为14.0%、27.7%、7.0%、6.2%。对羟基自由基的清除,水溶粗多糖在0.9 mg/ml时有最大清除率,为54.3%,碱溶纯多糖在0.06 mg/ml时有最大清除率,为10.9%;对超氧阴离子自由基的清除,水溶粗多糖在0.1mg/ml时有最大清除率,为24.2%,水溶纯多糖在0.07 mg/ml时有最大清除率,为27.2%。[结论]水溶多糖比碱溶多糖抗氧化能力强,桦褐孔菌可成为一种新的高效天然抗氧化药用真菌。

[Objective] This study aimed to explore the effects of different extraction methods on antioxidant activities of I.obliquus polysaccharides.[Method] Crude polysaccharides were extracted by using boiling ethanol precipitation method and ethanol-alkali extraction method;water-soluble and alkali-soluble polysaccharides were purified by using trichloroacetic acid method;antioxidant activities of I.obliquus polysaccharides were compared by hydroxyl radical scavenging experiment and superoxide anion radical scavenging experiment.[Result] Yield of crude water-soluble polysaccharides,crude alkali-soluble polysaccharides,pure water-soluble polysaccharides and pure alkali-soluble polysaccharides were 14.0%,27.7%,7.0% and 6.2%,respectively.Hydroxyl radical scavenging effect of crude water-soluble polysaccharides and pure alkali-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0.9 mg/ml and 0.06 mg/ml,and the scavenging rates were 54.3% and 10.9%,respectively;superoxide anion radical scavenging effect of crude water-soluble polysaccharides and pure water-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0.1 mg/ml and 0.07 mg/ml,and the scavenging rates were 24.2% and 27.2%,respectively.[Conclusion] Antioxidant activity of water-soluble polysaccharides was higher than that of alkali-soluble polysaccharides.I.obliquus could be used as a species of new efficient natural antioxidant medicinal fungi in application.