介孔分子筛固定化海藻糖合酶的研究

Research on Immobilization of Trehalose Synthase on Mesoporous Molecular Sieve

[目的]对介孔分子筛固定化海藻糖合酶进行研究。[方法]以基因工程菌所产海藻糖合成酶为材料,利用介孔分子筛MCM-41作为载体固定化海藻糖合成酶,并对固定化条件和固定化前后的海藻糖合酶性质进行比较研究。[结果]海藻糖合成酶可通过物理吸附法固定于介孔分子筛MCM-41孔道中,在pH为3,给酶量为62.5 mg/g的条件下,固定12 h时可得到最佳固定化效果;与游离海藻糖合酶相比,固定化后的海藻糖合酶pH稳定性和热稳定性明显改善,并具可重复操作性,有利于酶的使用和储放。[结论]该研究为开发新的固定化海藻糖合成酶提供了理论依据。

[Objective] This study aimed to explore the immobilization of trehalose synthase on mesoporous molecular sieves.[Method] With trehalose synthase produced by genetically engineered bacteria as experimental material,mesoporous molecular sieve MCM-41 was used as a carrier for immobilization of trehalose synthase.Properties of trehalose synthase before and after immobilization were compared.[Result] Trehalose synthesis could be immobilized on mesoporous molecular sieve MCM-41 by using physical adsorption method.Immobilization of trehalose synthase achieved the best effect with pH 3,enzyme amount of 62.5 mg/g and immobilization duration of 12 h.Compared with the free trehalose synthase,immobilized trehalose synthase showed significantly improved pH stability and thermal stability with repeatable operation,which was conducive to the utilization and storage of enzyme.[Conclusion] This study provided a theoretical basis for the development of new immobilized trehalose synthase.