海洋放线菌WBF16次级代谢产物的分离与结构鉴定

Isolation and identification of secondary metabolites from marine-derived actinomycete WBF16

目的分离和鉴定海洋来源的放线菌WBF16代谢产物中的抗肿瘤活性成分。方法利用大孔树脂、Sephadex LH-20凝胶柱层析、硅胶柱层析、反相柱色谱和HPLC等方法对该放线菌发酵产物进行分离,根据理化性质和波谱学方法进行化学结构的鉴定;利用MTT法来检测化合物的抗肿瘤活性。结果从海洋放线菌代谢产物中分离得到其特征代谢产物色霉素A2(1)和2-甲基-5,6,7-三甲氧基-1,4-萘醌(2)。结论化合物2为新天然产物,化合物1为首次从海洋微生物中分离得到,同时化合物1对人口腔上皮癌KB细胞、人肺癌细胞株A549、人肝癌细胞株SMMC-7721的细胞毒活性较好,IC50值分别为3.81、7.21和12.58μg/L。

Objective To isolate and identify the antitumor constituents from secondary metabolites of the marined-derived actinomycete WBF16. Methods The active fraction was isolated and purified from secondary metabolites of actinomycete WBF16 by macroporous resin, Sephadex LH-20 gel column chromatography, silica gel chromatography and preparative HPLC methods. Their structures were determined by spectral methods and biological activities were assayed by MTT method. Results Two compounds （1 and 2） were isolated and identified as chromamycine A2 and 2-methyl-5, 6, 7-methoxy-1, 4-naphthalene quinones. Conclusion Compound 2 was a new natural compound and compound 1 was firstly isolated from marine microorganisms. Compound 1 displayed better activity towards human oral epithelial carcinoma KB cells, human lung cancer cell line A549, human hepatocellular carcinoma cell line SMMC-7721, their IC50 values was 3.81, 7.21 and 12.58 ug/L, respectively.