蝙蝠蛾被孢霉多糖的分离纯化及理化性质

Extraction and characterization of polysaccharides from Mortierella hepiali

目的提取蝙蝠蛾被孢霉多糖,并对其理化性质进行研究。方法利用水提醇沉法得到粗多糖CPSM,采用硫酸-蒽酮法测定粗多糖含量,考马斯亮蓝法测定蛋白质含量,薄层色谱法检查是否含有单糖。CPSM经纤维素DE-52柱分离得CPSM-1和盐洗脱多糖。两种多糖分别经Sephadex系列凝胶柱分离纯化得到CPSM-1a和CPSM-2,采用光谱法和色谱法测定其单糖组成、分子质量等。结果粗多糖CPSM糖含量为61.4%,蛋白含量为1.8%,不含还原糖。CPSM-1a主要含有甘露糖、半乳糖和葡萄糖,其摩尔比约为5∶2∶3;CPSM-2主要含有甘露糖、半乳糖醛酸、半乳糖、葡萄糖和木糖,其摩尔比约为5∶1∶2∶1∶1。CPSM-1a和CPSM-2重均分子质量分别为8.8×103和7.6×103。结论 CPSM-1a为一水溶性中性多糖,CPSM-2为一水溶性酸性多糖。

Purpose To study the isolation and purification of polysaccharide from Mortierella hepialid. MethodsThe raw polysaccharide（CPSM） was extracted with water and the content of total of polysaccharide was determined with anthrone-sulfuric acid.Protein was determined by Bradford.Monosaccharide was detected by thin-layer chromatography.Two fractions of polysaccharide were obtained by Cellulose DE-52 column chromatography,then were purified respectively by Sephadex column chromatography to obtain CPSM-1a and CPSM-2.Physicochemical Properties of the two polysaccharides were studied by HPLC,FTIR,UV,GPC analysis.ResultsThe content of total polysaccharide,and protein in CPSM was 61.4% and 1.8% respectively,and monosaccharide was not detected.CPSM-1a was composed of Man,Gal,Glc with a molar ratio of 5∶2∶3.CPSM-2 was composed of Man,GalUA,Gal,Glc and Xyl with a molar ratio of 5∶1∶2∶1∶1.The weight-average molecular weight of CPSM-1a and CPSM-2 were estimated to be 8.8×103,7.6×103 respectively.ConclusionCPSM-1a is a neutral polysaccharide,and CPSM-2 is an acidic polysaccharide.