高效纤维素降解菌群的构建及其生物多样性分析

Construction of a composite microbial system for efficient cellulose degradation and its biodiversity analysis

以红树林植物根际富含生物质的土壤为材料,经过多代淘汰,筛选构建了1组纤维素降解复合菌群SYF。30℃静置培养10 d后,复合菌群对木榄、滤纸、红海榄的分解率分别为83.6%、71.5%和67.3%,纤维素酶活分别为102.2、126.3和110.7 U/mL。利用平板法分离得到了8个属的好氧性细菌和3个属的真菌,利用16S rDNA文库共检测到6个已知属和2个未知种类细菌,同时通过ITS文库共发现5个属真菌。仅有3个属的微生物可通过平板法和克隆文库构建法共同检测。

A composite microbial system SYF with efficient cellulose-degrading capacity was constructed from rhizosphere soil of mangrove plant rich in bio-substances by limited generation selections.It was found that 83.6% of Bruguiera gymnorrhiza leaves,71.5% of Rhizophora stylosa leaves and 67.3% of filter paper were degraded by the composite microbial system at 30 ℃ within 10 days under static culture conditions with the CMCase activity of 102.2,126.3 and 110.7 U/mL respectively.The microorganism diversity of SYF was analysis by the plate screening method and clone library establishing method.Eight genera of bacteria and 3 genera of fungi were isolated from the composite microbial system.Six kind of bacteria with known genera and two with unknown genera were detected by the 16S rDNA libraries,while 5 species of fungi were detected by the ITS libraries.Only 3 genera of the microorganisms were detected by both methods.