利用基因芯片研究植物非生物逆境响应基因表达的进展

Progress of microarray analysis for studying plant gene expression in response to abiotic stress

极端温度、干旱和高盐等逆境胁迫影响作物的正常生长,会导致作物大幅度减产。分子遗传和基因组学研究表明,大量基因受到逆境胁迫的诱导,包含转录因子在内的许多信号因子参与了逆境响应。基因芯片能够进行整个基因组范围的基因表达分析,利用基因表达谱分析,结合代谢组学和蛋白组学研究,已在阐明植物抗逆机制和发掘植物逆境胁迫响应相关基因方面取得重要进展。综述利用基因芯片对植物在极端温度、干旱和高盐等非生物逆境胁迫下基因表达的研究进展。

Extreme temperature,drought and high salinity are common stress conditions that affect both plant growth and crop production.Molecular and genomic studies have shown that expression of many genes with various functions are induced by extreme temperature,drought and high salinity,and various signaling factors including transcription factors are involved in the stress responses.Gene chip,a microarray-based expression profiling method which profiles the expression of genes in the whole genome,combined with metabonomics and proteomics has allowed significant progress in the characterization of plant genes related to stress response and in the elucidation of the underlying anti-stress mechanisms.In this review,we highlight the advances of microarray analysis of stress condition related genes in plants.