香蕉皮水溶性膳食纤维的提取及抗氧化研究

Soluble Dietary Fiber Extracted from Banana Peel and Its Antioxidant Activity

我国香蕉皮大部分没有得到利用,既浪费又产生环境污染。本文以香蕉皮为原料,采用超声波辅助酸水解法提取水溶性膳食纤维。通过单因素试验,考察了不同种类的酸、超声时间、料液比、提取液浓度、提取温度和时间对提取率的影响。在此基础上,采用L16(45)正交试验优化提取工艺。结果表明:最优工艺条件是以磷酸为提取液、料液比1:15(g/mL)、超声时间15 min、提取液浓度4%、提取温度80℃、提取时间120 min;在此条件下所获样品,提取率为17.97%,持水率5.82 g/g,持油力2.3 g/g,溶胀力6.31 mL/g。获得的水溶性膳食纤维对DPPH.和超氧自由基(O2-.)清除率分别达到47.51%和42.67%。

Banana peel from food industry in China is usually not used, which is not only wasteful but also pollutional. Extraction of soluble dietary fiber from banana peel was exploited by ultrasonic wave assisted acid hydrolysis method. Single factor experiments were carried out to determine the effects of acid type, ultrasonic treatment time, material-liquid ratio, acid concentration, extraction temperature and time on the extraction rate and a L16（45） orthogonal experiment was further conducted to optimize these extraction parameters. The results indicate that the optimal extraction condition was to extract at 80℃（2 for 120 rain after ultrasonic treatment for 15 min using 4% phosphoric acid as an extracting solution at l：l 5（g/mL） of material-liquid ratio. The extraction rate under this condition was up to 17.97% and the water-holding capacity, oil-holding capacity and swelling power of the extracted water-soluble dietary fiber were 5.82g/g, 2.3g/g and 6.3 l mL/g, respectively. The clearance rate of the fiber on DPPH. and superoxide radical （O2-＇） were 47.5 I% and 42.67%, respectively