4种黄酮类化合物对CCl_4致小鼠肝纤维化的防治作用

Inhibitory effects of four flavonoids on CCl_4 induced liver fibrosis in mice

目的探讨芹菜素、根皮苷、原花青素和新橙皮苷二氢查尔酮等黄酮类化合物在CCl4诱导的小鼠肝纤维化模型中的抗肝纤维化作用。方法将♂昆明种小鼠105只随机均分为正常对照组、CCl4模型组、秋水仙碱组、芹菜素组、根皮苷组、原花青素组、新橙皮苷二氢查尔酮组。给药5周后采血并处死小鼠,测定并比较各组小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的活性,总蛋白(TP)、白蛋白(ALB)及肝组织中的羟辅氨酸(HYP)的含量,计算小鼠的肝指数,观察肝脏的病理变化。结果 4种黄酮类化合物均可使模型小鼠血清中ALT、AST的含量、肝组织中羟脯氨酸的含量降低;病检也证明各组小鼠的肝细胞变性、坏死和肝纤维化均有不同程度的减轻。结论芹菜素、根皮苷、原花青素及新橙皮苷二氢查尔酮有抗CCl4致小鼠肝纤维化的作用。

OBJECTIVE To study the effect of four flavonoids（apigenin,phloridzin,oligomeric proanthocyanidins and neohesperidin dihydrochalcone） on anti-hepatic fibrosis in mice.METHODS The experimental model mice of hepatic fibrosis was induced by CCl4.105 male Kunming mice were randomly divided into 7 groups：normal control group,model group,colchicines group,apigenin group,phloridzin group,oligomeric proanthocyanidins group and neohesperidin dihydrochalcone group.All of them were sacrificed after five weeks of administration.The serum levels of ALT,AST,TP,ALB,HYP and liver index were determined.Liver tissue was handled with HE and Masson staining for hispathological study.RESULTS The four flavonoids significantly reduced the serum levels of ALT,AST,HYP and liver index.Histopathological observations showed that the four flavonoids could ameliorate the liver fibrosis in different degree.CONCLUSION The study showed that apigenin,phloridzin,oligomeric proanthocyanidins and neohesperidin dihydrochalcone had effect on preventing hepatic fibrosis.