癫痫平片中药材的鉴别及β-细辛醚的测定

Identification of medicinal materials in Dianxianping tablets and determination of β-asarone

目的建立完善癫痫平片的质量控制标准。方法用薄层色谱法鉴别癫痫平片中柴胡、猪牙皂,用HPLC法测定方中君药石菖蒲有效成分β-细辛醚的含量。结果薄层鉴别中,柴胡和猪牙皂供试品分别在与其对照药材相对保留时间的相同位置上,显相同颜色的斑点,且阴性无干扰;HPLC法测得β-细辛醚进样量0.212～2.65μg与峰面积的线性关系良好,回归方程为:Y=2.045×103X+10.430(r=0.9999),平均回收率为96.7%,RSD=1.08%。结论所用方法简便可行、重复性好,可用于癫痫平片的质量控制。

OBJECTIVE To establish and optimize the quality control standard of Dianxianping tablets.METHODS The medicinal herb of Bupleuri Radix and Gleditsiae Fructus were identified by TLC.HPLC was used to determine the content of β-asarone in Acod Talarinowii Rhizoma.RESULTS The results of TLC showed that there were spots with the same color at the same position between the tested medicinal material and the control of Bupleuri Radix and Gleditsiae Fructus,and blank sample had no interference.The results of HPLC showed that a good linearity was in the range of 0.212-2.65 μg in β-asarone,the regression equation was Y=2.045×103X＋10.430（r=0.9999） and the average recovery was 96.7%（RSD=1.08%）.CONCLUSION The method is simple and reproducible,and can be used for the quality control of Dianxianping tablets.