石斛合剂对HepG2细胞葡萄糖激酶与丙酮酸脱氢酶活性的影响

Dendrobium compound effects the activity of glucokinase and pyruvate dehydrogenase on HepG2 cell

目的探讨石斛合剂促进葡萄糖代谢的酶学机制。方法①以5%、10%、15%石斛合剂的含药血清培养HepG2细胞24h,测定葡萄糖激酶、丙酮酸脱氢酶活性;②以高浓度胰岛素培养HepG2细胞24h,继10%含药血清干预24h,测定葡萄糖激酶、丙酮酸脱氢酶的活性。结果石斛合剂的含药血清能提高HepG2细胞葡萄糖激酶、丙酮酸脱氢酶的活性(P<0.05);高浓度胰岛素能降低葡萄糖激酶、丙酮酸脱氢酶活性(P<0.05),石斛合剂的治疗能增加葡萄糖激酶、丙酮酸脱氢酶的活性(P<0.05)。结论高浓度胰岛素能诱发胰岛素抵抗,石斛合剂能提高葡萄糖代谢关键酶活性,缓解胰岛素抵抗。

OBJECTIVE To investigate the mechanism of the Dendrobium Compound（DC） improves glucose metabolism.METHODS ①Incubated HepG2 cells with 0、5%、10%、15% serum containing DC for 24h;②Incubated HepG2 cells with high concentration insulin for 24h to develop insulin resistance,followed by 10% serum containing DC for 24h,the activity of glucokinase and pyruvate dehydrogenase were measured.RESULTS The serum containing DC improves the activity of glucokinase and pyruvate dehydrogenase（P0.05）.High concentrations of insulin can reduce the activity of glucokinase and pyruvate dehydrogenase.And DC treatment can increase the activity of glucokinase and pyruvate dehydrogenase（P0.05）,reversing the downward effect of high insulin.CONCULSION High concentration of insulin induces insulin resistance in HepG2 cells,where the serum containing DC improves glucose metabolism by enhancing the activity of key enzymes involved in glucose metabolism,and alleviates insulin resistance.