EGCG-O-甲基转移酶(EOMT)催化EGCG形成的EGCG甲基化衍生物分析

Analysis on the EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase

研究了EGCG3′′Me、EGCG4′′Me和EGCG3′Me在正离子模式下的质谱裂解规律,并在此基础上分析了EGCG-O-甲基转移酶(EOMT)催化EGCG形成的反应产物。结果表明,EGCG-O-甲基转移酶催化EGCG后反应体系中生成了10余种不同的EGCG甲基化衍生物,主要包括EGCG3′′Me、EGCG4′′Me、EGCG3′Me、EGCG5′Me、EGCG3′′,4′′-diMe、EGCG3′′,5′′-diMe、EGCG3′,5′-diMe、EGCG3′,5′′-diMe、EGCG3′,3′′,5′′-triMe,以及EGCG5′,3′′,5′′-triMe等。

The fragmentation pathways of EGCG3＂Me, EGCG4＂Me and EGCG3＇Me were studied using LC-MS under positive ionization mode, and the EGCG methylated derivatives produced from EGCG catalyzed by EGCG-O-Methyltransferase were analyzed. Results showed that more than ten kinds of EGCG methylated derivatives were identified, including EGCG3＂Me, EGCG4＂Me, EGCG3＇Me, EGCG5＇Me, EGCG3＂,4＂-diMe, EGCG3＂,5＂-diMe, EGCG3＇,5＇-diMe, EGCG3＇,5＂-diMe, EGCG3＇,3＂,5＂-triMe, EGCG5＇,3＂,5＂-triMe