信号蛋白Smad1对2型糖尿病肾病诊断意义的初步研究

Evaluation of Signal Protein Smad1 in Diagnosis of Diabetic Nephropathy in Type 2 Diabetic Patients

目的探讨信号蛋白Smad1在2型糖尿病肾病诊断中的价值。方法选择我院2型糖尿病患者50例,根据24 h尿微量白蛋白定量分为2组:糖尿病无肾病组27例和糖尿病肾病组23例;同期相匹配的健康体检者24例为正常对照组,用酶联免疫吸附法(ELISA)测定所有受试者血和尿中Smad1的水平。结果血Smad1在正常对照组为41.4(32.8,66.7)ng/L,糖尿病无肾病组为23.6(19.7,29.3)ng/L,糖尿病肾病组为24.3(22.3,25.9)ng/L。尿Smad1在正常对照组32.7(29.1,36.7)ng/L,糖尿病无肾病组24.3(17.0,32.1)ng/L,糖尿病肾病组19.1(13.8,23.6)ng/L。血和尿Smad1在糖尿病无肾病组和糖尿病肾病组均低于正常对照组,差异有统计学意义(P<0.05),但在糖尿病无肾病组和糖尿病肾病组间差异无统计学意义(P>0.05)。多元线性回归分析显示,血和尿Smad1与肾功能相关指标均无相关性。结论信号蛋白Smad1可能不是2型糖尿病肾病诊断中一个有价值的指标。

Objective To investigate the evaluation of Smad1 in diagnosis of type 2 diabetic nephropathy.Methods Fifty type 2 diabetic patients in our hospital were divided into two groups by the content of 24-h urinary microalbumin：normal microalbumin（NC） group（n=27） and mass microalbumin（DN） group（n=23）.Meanwhile 24 health people were chosen as the normal control group.Serum and urinary Smad1 were determined with ELISA.Results The serum Smad1 content was 41.4（32.8,66.7）ng/L in the NC,23.6（19.7,29.3）ng/L in DM and 24.3（22.3,25.9）ng/L in DN.The urinary Smad1 content was 32.7（29.1,36.7）ng/L in NC,24.3（17.0,32.1） ng/L in DM and 19.1（13.8,23.6）ng/L in DN.Both of Serum and urinary Smad1 content in diabetic groups were lower than those in NC（P〈0.05）.There was no statistical significant difference between DM and DN（P〉0.05）.There was no correlation between the levels of Smad1 and renal-related indicators in diabetic patients by multi-linear regression model analysis.Conclusion The Smad1 could not be a valuable marker in diagnosis of diabetic nephropathy.