一种真鲷球形病毒的形态及细胞病理学电子显微镜观察

Electron microscope obseration on the morphology of a sphereovirus and cell pathology of Pagrosomus major

对 1 995年青岛地区患病真鲷肝脏、肠上皮组织、肾脏、鳃等组织进行电镜观察 ,发现一种球形病毒 ,该病毒具有囊膜 ,直径为 80～ 1 0 0nm ,在核和细胞质中均形成不规则形态包涵体 ;细胞病理变化表现为核内染色质变性 ,核膜水肿变形且部分溶解 ,内质网膨胀 ,胞质内有大量游离核糖体分布 ,线粒体外膜及内嵴变形且有少量溶解。

The report made use of the electron microscopy technology to examine the sick Pagrosomus Major at Qingdao in 1995 expounded, a lot of sphereovirus particles which were found in the cytonucleus and cytoplasm of tissues of hepar, gut, kidney and grill. Study result showed: the sphereovirus had an irregular inclusion body in cytonucleus and cytoplasm at all, whose diameter was between about 80-100nm.The virus particles had a middle density nucleus with an envelope. In diseased cells, nuclear chromatin changed and formed virus inclusion body,and part of the nuclear envelope was dissolved and distributed a lot of ribosome's over the cytoplasm, part of the outer membrane and crest of mitochondrion were out of shape and dissolved.