氨基葡萄糖盐酸盐的制备及其某些性质

Preparation and some properties of glucosamine hydrochloride

采用浓盐酸水解法从甲壳素制备氨基葡萄糖盐酸盐 ,经离子交换柱层析纯化后 ,以吡啶∶乙酸乙酯∶水∶冰醋酸 (5∶5∶3∶1 ,V/V)为展层剂进行纸层析鉴定 ,其Rf 值为 0 .44。氨基葡萄糖盐酸盐重新溶于水后比旋光度随时间而变化 ,从 1 0 0°降至 72°左右 ,2小时后处于稳定 ,[α]2 0D 为 71 .9°。经化学测定 ,氯离子含量为1 6 .74%。红外光谱显示 ,在 330 7.8cm-1、30 98.9cm-1、1 6 1 5 .0cm-1、1 5 34 .1cm-1、1 4 1 7.9cm-1、1 0 93.8cm-1、1 0 33.6cm-1、91 2 .0cm-1和 5 79.1cm-1处有特征吸收峰。

Glucosamine hydrochloride was prepared by hydrolysis of chitin with concentrated hydrochloric acid and then chromatographed on 732 cation exchange column for purification. The purified glucosamine hydrochloride was identified by paper chromatography using the solvent system of pyridine, ethyl acetate, water and glacial acetic acid (5∶5∶3∶1) and its Rf value was 0.44. On redissolving in water, the value of the specific optical rotation of glucosame hydrochloride decreased with time from nearly 100° to about 72°, and was stable after 2 hrs with [α] 20 D = 71.9°.The chloride content of glucosamine hydrochloride product was 16.74%.Its infrared spectrum shows that the characteristic absorption peaks occured at 3307.8cm -1 、3098.9cm -1 、1615.0cm -1 、1534.1cm -1 、1417.9cm -1 、1093.8cm -1 、1033.6cm -1 、912.0cm -1 and 579.1cm -1 .