拟南芥雄性不育突变体fne的遗传与定位分析

Genetic and Mapping Analysis of Arabidopsis thaliana Male Sterile Mutant filament no elongation

在T-DNA插入突变体Salk_118481株系的群体中,筛选到一株雄性不育突变体,用T-DNA序列上的一对引物进行PCR鉴定表明其基因组中没有T-DNA插入。通过背景纯化与遗传分析发现该雄性不育突变体是由单个隐性基因控制的,引起不育的主要原因是在花药发育的第13～14期,花丝不能伸长以完成授粉,故该突变体命名为fne(filament no elongation)。利用图位克隆的方法对FNE基因进行了定位,结果表明FNE基因位于第五条染色体上分子标记MBD2和MMG4之间的97kb区间内。目前该区间内尚未见到控制花丝伸长基因的报道,因此,FNE基因是一个控制花丝伸长的新基因。

To identify new genes important for anther development, we screened for malesterile mutants among a population of Arabidopsis ecotype Columbia （Col） mutagenized by TDNA insertion （provided by ARBC）. A male sterile mutant line with normal vegetative and flora development but no seed yield was isolated from Salk_118481 line. TDNA insertion site identification showed that there were no TDNA sequences in the genome of the mutants. Genetic analysis indicated that the mutant was controlled by a single recessive nuclear gene named filament no elongation because the filament of the mutant remains very short at the 13-14 stage of anther development. The fne gene was mapped to a region of 97kb between the molecular makers MBD2 and MMG4 on chromosome 5 using mapbased cloning technique. No genes involved filament elongation were reported in this region, so we believe that FNE gene could be a new gene controlling filament elongation in Arabidopsis.