微电极阵列芯片的脂质体电融合研究

Study of Liposome Electrofusion on Microelectrode Array Chip

利用旋转蒸发法制作基于大豆卵磷脂的一种大型脂质体,在微电极阵列芯片上进行脂质体电融合实验研究。在电融合过程中,利用介电电泳力实现脂质体在微流控芯片中的排队,再利用高场强的电脉冲使脂质体膜发生可逆性电穿孔,在持续的介电电泳力作用下,使穿孔的脂质体实现融合。芯片上脂质体的融合率可以达到20%左右。而且,玻璃基底材料和低深宽比的通道结构更有利于脂质体融合过程的观察与控制。

Large liposomes that electrofused on a microelectrode array based microchip were prepared by using soybean lecithin and the co-evaporating method.In the electrofusion process,liposomes were aligned by the dielectrophoretic force,electroporated by using high-strength electric pulses,and subsequently fused by using continuous dielectrophoretic force.Experimental results showed that 20% liposomes could be fused on the chip,and the glass substrate and low depth-width ratio channel were helpful for the observation and control of the liposome fusion.