中国流行性出血热自然疫源地结构和传播机理研究 Ⅵ.流行性出血热病毒感染黑线姬鼠和褐家鼠后病毒血症和分泌物、排泄物中带病毒情况实验观察

Studies on the Structure of Natural Foci of Infection and Mechanism of Transmission of Epidemic Hemorrhagic Fever(EHF)in China .Ⅵ. Experimental Observations on Vremia and Excretion of Virus in Secretions and Excretions after Infection of Apodemus

流行性出血热(EHF)病毒感染黑线姬鼠和褐家鼠实验结果表明:经肌肉和腹腔接种病毒,褐家鼠可于第7～9天检出肺抗原,第9～17天检出血清IgG抗体;接种病毒后第1天即可出现病毒血症,且持续到第10天。黑线姬鼠可于第5～15天检出肺抗原,第5天开始检出血清IgG抗体;接种病毒后第5天出现病毒血症,同时唾液中带有病毒。该实验未证实鼠尿、粪带有病毒,有待重复实验。

This experiment is meant to solve the Approaches of transimission of the virus of EHF in population of principal host animals of EHF in China, in order to make a further study and explanation of the route of'the human beings being infected by mice', we have performed an experimental study in the Gnotoflex Isolalor in our lab. The Result of experiment indicates that Apodemus agrarius and Rattus norvegicus, can be infected by all viral strains from the different sources (Zun Yi Hou Kan's strains from Apodemus agrarius and R_(26)'s strains from Yong Chun's Rattus norvegicus) and the different infectious routes (aerosal, feeding, nasal drip, scarification). From titer produced by experimental mice's specific IgG antibody, we know that Apodemus agrarius and Rattus norvegicus are easier to be infected through aerosal, Apodemus agrarius and Rattus norvegicus are more clearly seen to be more sensitive to the viral strains from own races. From titer of IgG antibody of EHF produced by Rattus norvegicus, we know that the titer produced by Rattus norvegicus is lower than that by Apodemus agrarius, whether this has something to do with the infective dose, requires to be studied further. This experiment proves further that these two kinds of experimental mice can be infected mutually with strains of both Apodemus agrartus and Rattus norveglcus. From this result, it is inferred that infectious areas of Apodemus type will gradually change into infectious areas of mixed type is the inevitable tendency. According to this, contral the number of Rattus norvegicus in an infectious area, not only can prevent infectious areas from being spreaded and enlarged but also will have important epidemtological significances for Blocking transmision from Apodemus agrarius to Rattus norvegicus.